Uros (NM_009479) Mouse Tagged ORF Clone

CAT#: MR203514

Uros (Myc-DDK-tagged) - Mouse uroporphyrinogen III synthase (Uros)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uros
• Synonyms: AI415298; UROIIIS; Uros3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203514 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTTCTCTTACTAAAAGACGCCAAGGAGGATGACAGCGGCCTGGATCCATATATCCAGGAGCTGC
  GATTGTGTGGCCTAGAAGCCACACTGATTCCTGTGCTGTCATTTGAGTTTATGTCTCTCCCCAGTTTGTC
  AGAAAAGCTGTCTCATCCTGAAGGCTTTGGAGGACTCATTTTCACCAGCCCCAGGGCAGTGGAAGCAGTG
  AAGCTGTGTTTGGAGAAGGACAATAAAACTGAAGCCTGGGAGAAGTCTCTGAAAGACAGATGGAATGCCA
  AGTCTGTGTACGTGGTTGGAAGTGCCACCGCTTCTCTAGTGAATAAAATTGGTCTGGATGCAGAAGGAGC
  GGGCAGTGGAAATGCAGAAAAGCTTGCTGAATATATTTGCTCAAAGCCATCTTCAGAGCTGCCTCTTCTC
  TTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGGATCCCCATGGAAA
  GCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCA
  GGGTATCCCAGCCAGCATCACGTTTTTCAGTCCCTCCGGCCTTAAATACAGCCTCGAGTATATTCAGGCG
  TTATCTGGCAGCAGCTTTGACCAGATTAAGTTTATAGCCATTGGCCCCAGTACAACCCGTGCTATGGCTG
  CTAAGGGCCTGCCTGTGAGCTGCACTGCAGAGAGCCCCACACCACAAGCCCTGGCTGCAGGCATCAGGAA
  TGTGCTGAAGCCAAACCACTGTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203514 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVLLLKDAKEDDSGLDPYIQELRLCGLEATLIPVLSFEFMSLPSLSEKLSHPEGFGGLIFTSPRAVEAV
  KLCLEKDNKTEAWEKSLKDRWNAKSVYVVGSATASLVNKIGLDAEGAGSGNAEKLAEYICSKPSSELPLL
  FPCGTIKGDTLPKMLKDKGIPMESMHVYQTVPHPGIQGSLKSYYEDQGIPASITFFSPSGLKYSLEYIQA
  LSGSSFDQIKFIAIGPSTTRAMAAKGLPVSCTAESPTPQALAAGIRNVLKPNHCC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009479
• ORF Size: 798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009479.1, NM_009479.2, NM_009479.3, NP_033505.1
• RefSeq Size: 1802 bp
• RefSeq ORF: 798 bp
• Locus ID: 22276
• MW: 28.5 kDa
• Gene Summary: The protein encoded by this gene is the fourth enzyme in the heme biosynthesis pathway. It converts hydroxymethylbilane to uroporphyrinogen III, a cyclic tetrapyrrole. This enzyme is defective in the autosomal recessive disorder congenital erythropoietic porphyria. Alternate promoter usage controls cell type-specific expression, including erythroid cell-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]