Uros (NM_009479) Mouse Tagged ORF Clone
CAT#: MR203514
- TrueORF®
Uros (Myc-DDK-tagged) - Mouse uroporphyrinogen III synthase (Uros)
"NM_009479" in other vectors (4)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Uros |
Synonyms | AI415298; UROIIIS; Uros3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203514 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTTCTCTTACTAAAAGACGCCAAGGAGGATGACAGCGGCCTGGATCCATATATCCAGGAGCTGC GATTGTGTGGCCTAGAAGCCACACTGATTCCTGTGCTGTCATTTGAGTTTATGTCTCTCCCCAGTTTGTC AGAAAAGCTGTCTCATCCTGAAGGCTTTGGAGGACTCATTTTCACCAGCCCCAGGGCAGTGGAAGCAGTG AAGCTGTGTTTGGAGAAGGACAATAAAACTGAAGCCTGGGAGAAGTCTCTGAAAGACAGATGGAATGCCA AGTCTGTGTACGTGGTTGGAAGTGCCACCGCTTCTCTAGTGAATAAAATTGGTCTGGATGCAGAAGGAGC GGGCAGTGGAAATGCAGAAAAGCTTGCTGAATATATTTGCTCAAAGCCATCTTCAGAGCTGCCTCTTCTC TTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGGATCCCCATGGAAA GCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCA GGGTATCCCAGCCAGCATCACGTTTTTCAGTCCCTCCGGCCTTAAATACAGCCTCGAGTATATTCAGGCG TTATCTGGCAGCAGCTTTGACCAGATTAAGTTTATAGCCATTGGCCCCAGTACAACCCGTGCTATGGCTG CTAAGGGCCTGCCTGTGAGCTGCACTGCAGAGAGCCCCACACCACAAGCCCTGGCTGCAGGCATCAGGAA TGTGCTGAAGCCAAACCACTGTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203514 protein sequence
Red=Cloning site Green=Tags(s) MKVLLLKDAKEDDSGLDPYIQELRLCGLEATLIPVLSFEFMSLPSLSEKLSHPEGFGGLIFTSPRAVEAV KLCLEKDNKTEAWEKSLKDRWNAKSVYVVGSATASLVNKIGLDAEGAGSGNAEKLAEYICSKPSSELPLL FPCGTIKGDTLPKMLKDKGIPMESMHVYQTVPHPGIQGSLKSYYEDQGIPASITFFSPSGLKYSLEYIQA LSGSSFDQIKFIAIGPSTTRAMAAKGLPVSCTAESPTPQALAAGIRNVLKPNHCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009479 |
ORF Size | 798 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009479.1, NM_009479.2, NM_009479.3, NP_033505.1 |
RefSeq Size | 1802 bp |
RefSeq ORF | 798 bp |
Locus ID | 22276 |
MW | 28.5 kDa |
Gene Summary | The protein encoded by this gene is the fourth enzyme in the heme biosynthesis pathway. It converts hydroxymethylbilane to uroporphyrinogen III, a cyclic tetrapyrrole. This enzyme is defective in the autosomal recessive disorder congenital erythropoietic porphyria. Alternate promoter usage controls cell type-specific expression, including erythroid cell-specific expression. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205297 | Uros (untagged) - Mouse uroporphyrinogen III synthase (Uros), (10ug) |
USD 210.00 |
|
MG203514 | Uros (GFP-tagged) - Mouse uroporphyrinogen III synthase (Uros) |
USD 300.00 |
|
MR203514L3 | Lenti ORF clone of Uros (Myc-DDK-tagged) - Mouse uroporphyrinogen III synthase (Uros) |
USD 500.00 |
|
MR203514L4 | Lenti ORF clone of Uros (mGFP-tagged) - Mouse uroporphyrinogen III synthase (Uros) |
USD 500.00 |
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