Zdhhc21 (NM_026647) Mouse Tagged ORF Clone

CAT#: MR203515

Zdhhc21 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 21 (Zdhhc21)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zdhhc21
• Synonyms: 9130404H11Rik; AL024349; D130004H04Rik; dep
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203515 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTTCGGATTCACTTTGTTGTTGACCCGCATGGTTGGTGCTGCATGGGCTTGATTGTCTTTGTCT
  GGTTATACAATATTGTTATAATTCCCAAAATTGTCCTCTTTCCTCACTATGAAGAAGGACATATTCCTGG
  CATTTTAATAATAATATTCTATGGCATTTCCATATTTTGCCTGGTTGCCTTAGTGAGGGCCTCCTTAACT
  GATCCTGGAAGACTCCCTGAAAATCCCAAGATCCCACATGCAGAGCGGGAGCTGTGGGAGCTCTGCAACA
  AGTGCAACCTGATGAGGCCGAAGCGCTCCCACCACTGCAGCCGCTGCGGCCACTGCGTCAGGAGGATGGA
  CCATCACTGTCCTTGGATAAACAATTGTGTGGGTGAAGACAACCATTGGCTCTTTCTGCAGTTGTGTTTC
  TACACTGAGCTGCTTACTTGCTACGCACTGATGTTTTCTTTCTGCCATTATTATTATTTTCTTCCACTAA
  AAAAGCGTAACTTGGATCTCTTTGTTGTTCGCCATGAGCTGGCTATCATGAGACTAGCTGCCTTCATGGG
  CATCACTATGTTAGTTGGAATAACTGGACTCTTCTATACACAACTGATTGGCATCATCACAGATACAACA
  TCTATTGAAAAAATGTCAAATTGTTGTGAAGAAATATCGCGGCCCCGAAAGCCATGGCAGCAGACCTTCT
  CAGAAGTTTTTGGCACTCGTTGGAAGATCCTGTGGTTCATTCCTTTCAGGCAGCGGCAACCACTGCGAGT
  TCCCTACCACTTTGCCAATCACGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203515 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLRIHFVVDPHGWCCMGLIVFVWLYNIVIIPKIVLFPHYEEGHIPGILIIIFYGISIFCLVALVRASLT
  DPGRLPENPKIPHAERELWELCNKCNLMRPKRSHHCSRCGHCVRRMDHHCPWINNCVGEDNHWLFLQLCF
  YTELLTCYALMFSFCHYYYFLPLKKRNLDLFVVRHELAIMRLAAFMGITMLVGITGLFYTQLIGIITDTT
  SIEKMSNCCEEISRPRKPWQQTFSEVFGTRWKILWFIPFRQRQPLRVPYHFANHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026647
• ORF Size: 798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026647.1, NM_026647.2, NM_026647.3, NP_080923.2
• RefSeq Size: 8751 bp
• RefSeq ORF: 798 bp
• Locus ID: 68268
• MW: 31.3 kDa