Il33 (NM_001164724) Mouse Tagged ORF Clone

CAT#: MR203528

Il33 (Myc-DDK-tagged) - Mouse interleukin 33 (Il33), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il33
• Synonyms: 9230117N10Rik; Il-33; Il1f11; NF-HEV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203528 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCTAGAATGAAGTATTCCAACTCCAAGATTTCCCCGGCAAAGTTCAGCAGCACCGCAGGCGAAG
  CCCTGGTCCCGCCTTGCAAAATAAGAAGATCCCAACAGAAGACCAAAGAATTCTGCCATGTCTACTGCAT
  GAGACTCCGTTCTGGCCTCACCATAAGAAAGGAGACTAGTTATTTTAGGAAAGAACCCACGAAAAGATAT
  TCACTAAAATCGGGTACCAAGCATGAAGAGAACTTCTCTGCCTATCCACGGGATTCTAGGAAGAGATCCT
  TGCTTGGCAGTATCCAAGCATTTGCTGCGTCTGTTGACACATTGAGCATCCAAGGAACTTCACTTTTAAC
  ACAGTCTCCTGCCTCCCTGAGTACATACAATGACCAATCTGTTAGTTTTGTTTTGGAGAATGGATGTTAT
  GTGATCAATGTTGACGACTCTGGAAAAGACCAAGAGCAAGACCAGGTGCTACTACGCTACTATGAGTCTC
  CCTGTCCTGCAAGTCAATCAGGCGACGGTGTGGATGGGAAGAAGGTGATGGTGAACATGAGTCCCATCAA
  AGACACAGACATCTGGCTGCATGCCAACGACAAGGACTACTCCGTGGAGCTTCAAAGGGGTGACGTCTCG
  CCTCCGGAACAGGCCTTCTTCGTCCTTCACAAAAAGTCCTCGGACTTTGTTTCATTTGAATGCAAGAATC
  TTCCTGGCACTTACATAGGAGTAAAAGATAACCAGCTGGCTCTAGTGGAGGAGAAAGATGAGAGCTGCAA
  CAATATTATGTTTAAGCTCTCGAAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203528 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRPRMKYSNSKISPAKFSSTAGEALVPPCKIRRSQQKTKEFCHVYCMRLRSGLTIRKETSYFRKEPTKRY
  SLKSGTKHEENFSAYPRDSRKRSLLGSIQAFAASVDTLSIQGTSLLTQSPASLSTYNDQSVSFVLENGCY
  VINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKVMVNMSPIKDTDIWLHANDKDYSVELQRGDVS
  PPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164724
• ORF Size: 801 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001164724.1, NP_001158196.1
• RefSeq Size: 2555
• RefSeq ORF: 801
• Locus ID: 77125
• MW: 30 kDa
• Gene Summary: In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProtKB/Swiss-Prot Function]