Med8 (NM_020000) Mouse Tagged ORF Clone

CAT#: MR203549

Med8 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) (Med8), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Med8
• Synonyms: 2210021A15Rik; AB041805; ARC32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203549 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGGGAGGAGAAGCAGCTTGAGGCATCATTAGATGCCCTGCTGAATCAAGTGGCCGATCTGAAGA
  ATTCACTGGGGAGTTTCATTTACAAGTTGGAAAACGAGTATGACCGGCTGACCTGGCCCTCTGTCCTGGA
  CAGCTTTGCATTGCTTTCTGGACAGTTGAACACTCTGAACAAGGTCTTAAAGCATGAGAAGACGCCACTG
  TTCCGAAACCAGGTCATCATCCCTCTGGTGCTGTCCCCAGACCGAGACGAAGACCTCATGCGTCAAACTG
  AAGGACGAGTGCCTGTTTTCAGCCATGAGGTCGTCCCTGACCACTTGAGAACCAAGCCTGACCCTGAAGT
  TGAAGAGCAGGAGAAGCAACTGACAACAGATGCTGCCAGAATTGGTGCTGATGCAGCTCAGAAGCAGATC
  CAGAGCTTGAATAAAATGTGCTCAAACCTTTTAGAGAAAATCAGCAAAGAAGAACGAGAATCAGAGAGCG
  GAGGTCTCCGGCCAAACAAGCAGACTTTCAACCCTGGAGACACCAATGCCTTAGTAGCAGCCGTTGCCTT
  TGGGAAGGGGCTATCTAATTGGCGACCTTCAGGTAGCAGTGGTCCTGGGCAACCCGGTCAGCCAGGAGCT
  GGTACAATCCTTGCAGGGGCCTCAGGATTACCGCAGGTTCAGATGCCAGGTGCTCCAAACCAGCAGCAGC
  CAATGCTCAGTGGAGTCCAGATGGCTCAGGCAGGTCAACCAGGGAAAATGCCAAGTGGAATAAAAACCAA
  CATCAAGTCAGCTTCAATGCATCCGTACCAGCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203549 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQREEKQLEASLDALLNQVADLKNSLGSFIYKLENEYDRLTWPSVLDSFALLSGQLNTLNKVLKHEKTPL
  FRNQVIIPLVLSPDRDEDLMRQTEGRVPVFSHEVVPDHLRTKPDPEVEEQEKQLTTDAARIGADAAQKQI
  QSLNKMCSNLLEKISKEERESESGGLRPNKQTFNPGDTNALVAAVAFGKGLSNWRPSGSSGPGQPGQPGA
  GTILAGASGLPQVQMPGAPNQQQPMLSGVQMAQAGQPGKMPSGIKTNIKSASMHPYQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020000
• ORF Size: 807 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020000.2, NM_020000.3, NP_064384.2
• RefSeq Size: 1887 bp
• RefSeq ORF: 807 bp
• Locus ID: 80509
• MW: 29.2 kDa