Plpp2 (NM_015817) Mouse Tagged ORF Clone

CAT#: MR203710

Ppap2c (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2C (Ppap2c)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Plpp2
• Synonyms: Lpp2; PAP2-G; PAP2-gamma; PAP2c; Ppap2c
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203710 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGAGGTGGGTCTTCGTGCTGCTGGACGTGCTGTGCGTGTTGGTCGCCTCTCTGCCTTTCATCA
  TCCTGACCCTGGTGAATGCACCATATAAGCGGGGGTTCTACTGTGGAGATGACTCCATCCGGTACCCATA
  CCGTCCAGACACGATCACCCACGGACTCATGGCTGGGGTCATCATCACAGCTACTGTCATCCTTGTCTCA
  TTGGGAGAAGCCTACCTGGTGTACACAGACCGTCTTTATTCGCGATCCAACTTCAACAACTATGTAGCTG
  CCATCTACAAGGTGCTGGGAACCTTTCTGTTCGGGGCTGCTGTGAGCCAGTCTCTCACCGACCTGGCCAA
  GTACATGATTGGCCGTCTTCGACCCAGTTTCTTGGCTGTCTGTGACCCTGACTGGAGCCAGGTCAACTGT
  TCTGGCTATGTGCAGCTGGAGGTGTGCAGGGGCAGCCCTGCTAATGTCACGGAGGCCAGGCTGTCCTTCT
  ACTCTGGCCACTCCTCCTTTGGCATGTATTGCATGTTGTTCCTGGCGCTATATGTGCAGGCCCGGCTCTG
  CTGGAAGTGGGCACGGCTGCTGAGGCCCACTGTTCAGTTCTTCTTGGTGGCCTTTGCAATCTATGTGGGC
  TATACCCGAGTGTCTGACCACAAGCACCACTGGAGTGATGTCCTTGTCGGCCTCCTGCAGGGAGCCCTGG
  TGGCCTGCCTCACGGTCCGCTATGTTTCAGATTTCTTCAAATCCCGGCCACCCCAGCCCTGCCAGGAGGA
  TGAAGTGCCGGAGCGCAAGCCCAGTCTGTCACTGACGCTGACCCTTGGTGACCGACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203710 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERRWVFVLLDVLCVLVASLPFIILTLVNAPYKRGFYCGDDSIRYPYRPDTITHGLMAGVIITATVILVS
  LGEAYLVYTDRLYSRSNFNNYVAAIYKVLGTFLFGAAVSQSLTDLAKYMIGRLRPSFLAVCDPDWSQVNC
  SGYVQLEVCRGSPANVTEARLSFYSGHSSFGMYCMLFLALYVQARLCWKWARLLRPTVQFFLVAFAIYVG
  YTRVSDHKHHWSDVLVGLLQGALVACLTVRYVSDFFKSRPPQPCQEDEVPERKPSLSLTLTLGDRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015817
• ORF Size: 831 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015817.2, NM_015817.3, NP_056632.2
• RefSeq Size: 1646 bp
• RefSeq ORF: 831 bp
• Locus ID: 50784
• MW: 31.2 kDa
• Gene Summary: The protein encoded by this gene is a lipid phosphate phosphohydrolase. It is an integral membrane protein that catalyzes the conversion of phosphatidic acid to diacylglycerol and inorganic phosphate. The transcript is expressed at high levels in lung, liver, and kidney and at low levels in brain and heart. Null mutant mice are viable and fertile and display no overt phenotypic defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]