F3 (NM_010171) Mouse Tagged ORF Clone

CAT#: MR204046

F3 (Myc-DDK-tagged) - Mouse coagulation factor III (F3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F3
• Synonyms: AA409063; CD142; Cf-3; Cf3; TF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204046 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATCCTCGTGCGCCCGCGCCTCCTAGCGGCTCTCGCGCCCACGTTTCTCGGCTGCCTCCTCCTCC
  AGGTGATCGCGGGTGCAGGCATTCCAGAGAAAGCGTTTAATTTAACTTGGATATCAACTGATTTCAAGAC
  AATTTTGGAGTGGCAACCCAAACCCACCAACTATACCTACACTGTACAGATAAGTGATCGATCTAGAAAC
  TGGAAAAACAAGTGCTTCTCGACCACAGACACCGAGTGCGACCTCACAGACGAGATCGTGAAGGATGTGA
  CCTGGGCCTATGAAGCAAAGGTCCTCTCTGTCCCACGGAGGAACTCAGTTCATGGAGACGGAGACCAACT
  TGTGATTCATGGGGAGGAGCCGCCATTTACAAACGCCCCAAAGTTTTTACCTTACCGAGACACAAACCTC
  GGACAGCCAGTAATTCAGCAGTTTGAACAAGATGGTAGAAAACTGAACGTGGTTGTAAAAGACTCACTTA
  CATTAGTCAGAAAGAATGGTACATTCCTCACCCTGCGGCAAGTCTTTGGCAAGGACTTGGGTTATATAAT
  TACTTATCGGAAAGGCTCAAGCACGGGAAAGAAAACAAACATTACAAACACCAATGAATTCTCGATTGAT
  GTGGAAGAAGGAGTAAGCTACTGCTTTTTTGTACAAGCTATGATTTTCTCCAGGAAAACTAACCAAAATA
  GCCCAGGAAGCAGTACAGTGTGCACCGAGCAATGGAAGAGTTTCCTGGGAGAAACACTCATCATTGTGGG
  AGCAGTGGTGCTCCTGGCCACCATCTTTATCATCCTCCTGTCCATATCTCTGTGCAAGCGCAGAAAGAAC
  CGAGCGGGACAGAAAGGGAAGAACACCCCGTCGCGCTTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204046 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAILVRPRLLAALAPTFLGCLLLQVIAGAGIPEKAFNLTWISTDFKTILEWQPKPTNYTYTVQISDRSRN
  WKNKCFSTTDTECDLTDEIVKDVTWAYEAKVLSVPRRNSVHGDGDQLVIHGEEPPFTNAPKFLPYRDTNL
  GQPVIQQFEQDGRKLNVVVKDSLTLVRKNGTFLTLRQVFGKDLGYIITYRKGSSTGKKTNITNTNEFSID
  VEEGVSYCFFVQAMIFSRKTNQNSPGSSTVCTEQWKSFLGETLIIVGAVVLLATIFIILLSISLCKRRKN
  RAGQKGKNTPSRLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010171
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010171.1, NM_010171.2, NM_010171.3, NP_034301.2
• RefSeq Size: 1876 bp
• RefSeq ORF: 885 bp
• Locus ID: 14066
• MW: 32.9 kDa
• Gene Summary: This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]