Klf7 (NM_033563) Mouse Tagged ORF Clone

CAT#: MR204201

  • TrueORF®

Klf7 (Myc-DDK-tagged) - Mouse Kruppel-like factor 7 (ubiquitous) (Klf7)


  "NM_033563" in other vectors (4)


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Reconstitution Protocol

USD 68.00

USD 149.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Klf7
Synonyms 9830124P08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204201 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTGTTGGCTAGTTATAGTATATTCCAGGAGCTACAACTTGTCCACGACACCGGCTACTTCTCAG
CTTTGCCATCCCTGGAGGAGACCTGGCAGCAGACATGCCTTGAGTTGGAACGCTACCTCCAGACAGAACC
CCGGCGGATCTCGGAGACCTTTGGTGAGGACTTGGACTGCTTCCTCCACGCTTCTCCTCCCCCATGCATT
GAGGAGAGCTTCCGGCGCCTAGACCCCTTGCTGCTCCCAGTGGAAGCCACCATCTGCGAGAAGAGCTCCG
CAGTGGACATTTTGCTCTCTCGGGACAAGTTGCTATCTGAGACCTGCCTCAGCCTCCAGCCAACCAGCTC
TTCTCTAGACAGCTACACAGCCGTCAACCAGGCCCAGCTCAACGCAGTGACCTCATTAACGCCGCCCTCA
TCCCCTGAGCTCAGCCGCCATCTGGTCAAAACCTCACAGACTCTCTCAGCCGTGGATGGCACAGTGACGT
TGAAACTGGTGGCCAAGAAGGCTTCCCTCAGCTCGGTGAAGGTGGGAGGGGTGGCGGCGGCGGCAGCCGT
GACACCAGCTGGAGCAGTTAAGAGTGGACAGAGCGACAGTGAGCAAGGAGGGGGAGGGGCTGACACATGC
CCTGAAAACAAGAAGAGGGTCCATCGCTGTCAGTTTAACGGGTGCCGGAAAGTTTATACAAAAAGCTCCC
ACTTAAAGGCCCACCAGAGGACTCACACAGGTGAGAAGCCTTACAAGTGCTCATGGGAAGGATGCGAGTG
GCGTTTTGCACGGAGCGATGAGCTCACAAGGCACTACAGGAAACACACAGGTGCAAAGCCCTTTAAGTGT
AACCACTGCGACAGGTGTTTCTCCAGGTCTGACCATCTTGCGCTCCATATGAAGAGACATATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204201 protein sequence
Red=Cloning site Green=Tags(s)

MDVLASYSIFQELQLVHDTGYFSALPSLEETWQQTCLELERYLQTEPRRISETFGEDLDCFLHASPPPCI
EESFRRLDPLLLPVEATICEKSSAVDILLSRDKLLSETCLSLQPTSSSLDSYTAVNQAQLNAVTSLTPPS
SPELSRHLVKTSQTLSAVDGTVTLKLVAKKASLSSVKVGGVAAAAAVTPAGAVKSGQSDSEQGGGGADTC
PENKKRVHRCQFNGCRKVYTKSSHLKAHQRTHTGEKPYKCSWEGCEWRFARSDELTRHYRKHTGAKPFKC
NHCDRCFSRSDHLALHMKRHI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033563
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033563.1, NM_033563.2, NP_291041.2
RefSeq Size 1344 bp
RefSeq ORF 906 bp
Locus ID 93691
MW 33.3 kDa
Gene Summary Transcriptional activator. Binds specifically to an Ikaros core binding element that is crucial for in vivo NTRK1/TrkA enhancer function. [UniProtKB/Swiss-Prot Function]

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