Hsd17b13 (NM_198030) Mouse Tagged ORF Clone

CAT#: MR204267

Hsd17b13 (Myc-DDK-tagged) - Mouse hydroxysteroid (17-beta) dehydrogenase 13 (Hsd17b13), transcript variant 2

Product Images

Coomassie blue staining of purified Hsd17b13 protein (Cat# TP504267). The protein was produced from HEK293T cells transfected with Hsd17b13 cDNA clone (Cat# MR204267) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd17b13
• Synonyms: AI047820; Pan1b; PAN1B-like
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204267 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCATCCTGGAATTTCTCCTTCTGGTGGGCGTCATCATCTACTCCTACCTGGAGTCACTGGTAA
  AGTTCTTCATTCCCCGGAGAAGGAAATCTGTGACCGGGCAGACCGTTCTCATCACGGGGGCCGGACACGG
  AATAGGCAGGCTGACTGCATATGAATTTGCAAAGCAGAAAAGCAGACTGGTTCTATGGGATATCAATAAG
  CGTGGTGTTGAGGAAACCGCGGACAAATGCAGGAAACTGGGGGCCGTCGTGCACGTGTTTGTGGTGGACT
  GCAGCAACCGGGCCGAGATTTACAACTCTGTGGATCAGGTAAAGAGAGAAGTAGGTGATGTCGAGATCGT
  GGTAAACAACGCCGGGGCGATATATCCAGCAGACCTTCTTAGTGCCAAGGACGAGGAGATCACCAAGACC
  TTTGAGGTCAATATCCTCGGACATTTTTGGATCATAAAAGCACTCCTTCCATCGATGCTGAGAAGAAACT
  CTGGCCACATTGTCACAGTGGCTTCGGTGTGCGGCCACGGAGTGATTCCTTATCTCATCCCTTATTGCTC
  CAGCAAGTTTGCTGCTGTGGGCTTCCACCGAGCACTGACCGCAGAACTGGACACCTTGGGGAAAACCGGT
  ATCAAAACCTCGTGTCTCTGCCCTGTGTTCGTGAATACTGGCTTCACCAAAAACCCGAGTACAAGGTTAT
  GGCCTGTATTAGAGCCGGAAGAAGTTGCAAGGAGTCTGATCAATGGAATACTTACCAACAAGAAAATGAT
  TTTCGTTCCATCCTATATCAATATTTTTCTGATCCTGGAAAAAGGACCTGGATTCAGTTCCAAGCACCCA
  CATGGTGGTTCTCAACAGCCTGTTACTCCAATCCCAGGGGATTTGACACCCTCTTCAGACTTTCTCAAAC
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204267 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNLILEFLLLVGVIIYSYLESLVKFFIPRRRKSVTGQTVLITGAGHGIGRLTAYEFAKQKSRLVLWDINK
  RGVEETADKCRKLGAVVHVFVVDCSNRAEIYNSVDQVKREVGDVEIVVNNAGAIYPADLLSAKDEEITKT
  FEVNILGHFWIIKALLPSMLRRNSGHIVTVASVCGHGVIPYLIPYCSSKFAAVGFHRALTAELDTLGKTG
  IKTSCLCPVFVNTGFTKNPSTRLWPVLEPEEVARSLINGILTNKKMIFVPSYINIFLILEKGPGFSSKHP
  HGGSQQPVTPIPGDLTPSSDFLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198030
• ORF Size: 915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198030.1, NM_198030.2, NP_932147.2
• RefSeq Size: 1730
• RefSeq ORF: 915
• Locus ID: 243168
• MW: 33.5 kDa