Ppp6c (NM_024209) Mouse Tagged ORF Clone

CAT#: MR204292

Ppp6c (Myc-DDK-tagged) - Mouse protein phosphatase 6, catalytic subunit (Ppp6c)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp6c
• Synonyms: 2310003C10Rik; Pp6C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204292 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCTGGATCTGGACAAGTATGTGGAGATAGCGCGGCAGTGCAAGTACCTTCCCGAGAACGACC
  TGAAGAGGCTATGTGACTATGTTTGTGATCTGCTCTTGGAAGAGTCGAATGTTCAGCCAGTGTCAACGCC
  AGTAACAGTGTGTGGTGACATACACGGACAGTTTTATGATCTTTGTGAACTGTTCAGAACTGGAGGTCAG
  GTTCCTGACACAAACTACATATTTATGGGTGATTTTGTAGACAGAGGTTACTATAGTTTGGAGACCTTCA
  CTTATCTTCTTGCACTAAAGGCTAAGTGGCCTGACCGTATTACACTTTTAAGAGGAAATCATGAGAGTAG
  ACAGATAACACAGGTGTATGGATTTTATGATGAGTGCCAAACCAAATATGGAAATGCTAATGCCTGGAGA
  TACTGTACCAAAGTTTTTGATATGCTCACAGTAGCAGCTTTAATAGATGAGCAGATTTTGTGTGTTCATG
  GCGGTTTATCTCCTGATATCAAAACACTGGATCAAATTCGAACTATTGAACGAAATCAGGAAATTCCTCA
  CAAAGGAGCATTTTGTGACTTGGTGTGGTCAGATCCTGAAGATGTGGACACTTGGGCAATCAGTCCCAGA
  GGAGCAGGTTGGCTATTTGGAGCAAAAGTCACAAATGAGTTTGTTCATATCAACAACTTAAAACTCATCT
  GCAGAGCACATCAGCTTGTGCACGAAGGCTATAAGTTTATGTTTGACGAGAAGCTGGTCACAGTGTGGTC
  TGCTCCTAACTACTGCTATCGCTGTGGAAACATTGCTTCCATCATGGTCTTCAAAGATGTCAATACGAGA
  GAACCAAAGTTATTCCGAGCAGTTCCAGATTCAGAACGTGTTATTCCTCCCAGAACCACGACGCCGTATT
  TCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204292 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPLDLDKYVEIARQCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQ
  VPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWR
  YCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPR
  GAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTR
  EPKLFRAVPDSERVIPPRTTTPYFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024209
• ORF Size: 918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024209.1, NM_024209.2, NP_077171.1
• RefSeq Size: 1583 bp
• RefSeq ORF: 918 bp
• Locus ID: 67857
• MW: 35.2 kDa