Lgals12 (NM_019516) Mouse Tagged ORF Clone

CAT#: MR204480

Lgals12 (Myc-DDK-tagged) - Mouse lectin, galactose binding, soluble 12 (Lgals12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lgals12
• Synonyms: gal-12; Grip1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204480 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACTGACGAACACCTGGACCCGATTCCTGACAGCTTCATCCTGCAGCCGCCAGTCTTCCACCCGG
  TGATTCCTTATGGCACAACAATTTTTGGTGGCCTGTATGCAGGCAAGATGGTCACGCTGCAGGGTGTGGT
  CCCTCTGCATGCAAGGAGGTTTCAGGTGGACTTCCAGTGTGGGTGCTGCCTGCATCCTCAGCCAGATGTT
  GCCTTCCGCTTCAGCCCTCGATTCTACACTGTCAAGCCCCATGTCATCTGCAACACCCACCAAGGTGGAC
  TCTGGCAAAAAGAGATCCGGTGGCCAGGGGTCGCCCTGCAGAGAGGGGATAGCTTCCTCATTCTCTTTCT
  CTTTGAGAACGAAGAGGTGAAGGTGAGTGTAAATGGCCAGCACTTTCTTCACTACCGCTACCGGCTCCCA
  CTGTCACGGGTAGATACCCTTGACATATCTGGTGACATCTTGGTAAAGGCTGTTGGATTCCTGAACATCA
  ATCCATTTGTGGAGGGTAGCAGAGAGTATCCAGTTGGATATCCCTTCCTGCTGTATAGCCCCAGGCTGGA
  GGTGCCCTGCTCACGTGCTCTTCCTCGGGGTCTCTGGCCTGGGCAAGTCATTGTAGTTCGAGGACTGGTC
  TTGAAAGAGCCGAAAGATTTTACCCTGAGCCTGAAGGATGGGACCACCCATGTTCCTGTGACACTCAGGG
  CTTCCTTCACAGACAGAACACTGGCCTGGGTCTCCTCCTGGGGACGAAAGAAGCTGATCTCCGCCCCCTT
  CCTCTTTCACCCCCAGCGATTCTTCGAGGTACTGCTTCTGTGCCAAAAGGGAGGGCTGAAGTTGGCACTC
  AATGGGCAGGGGCTGGGGGCCACCAGCCTGGACCAGAAAGCCCTGGAGCAGCTGCGGGAGCTCAGGATCA
  GTGGAAATGTCCACCTCTACTGTGTCCACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204480 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTDEHLDPIPDSFILQPPVFHPVIPYGTTIFGGLYAGKMVTLQGVVPLHARRFQVDFQCGCCLHPQPDV
  AFRFSPRFYTVKPHVICNTHQGGLWQKEIRWPGVALQRGDSFLILFLFENEEVKVSVNGQHFLHYRYRLP
  LSRVDTLDISGDILVKAVGFLNINPFVEGSREYPVGYPFLLYSPRLEVPCSRALPRGLWPGQVIVVRGLV
  LKEPKDFTLSLKDGTTHVPVTLRASFTDRTLAWVSSWGRKKLISAPFLFHPQRFFEVLLLCQKGGLKLAL
  NGQGLGATSLDQKALEQLRELRISGNVHLYCVHC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019516
• ORF Size: 945 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019516.1, NM_019516.2, NM_019516.3, NP_062389.1
• RefSeq Size: 2755 bp
• RefSeq ORF: 945 bp
• Locus ID: 56072
• MW: 35.5 kDa
• Gene Summary: Binds lactose. May participate in the apoptosis of adipocytes. [UniProtKB/Swiss-Prot Function]