Rdh11 (NM_021557) Mouse Tagged ORF Clone

CAT#: MR204536

Rdh11 (Myc-DDK-tagged) - Mouse retinol dehydrogenase 11 (Rdh11)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rdh11
• Synonyms: 2610319N22Rik; AI428145; Arsdr1; AU045252; C85936; CGI-82; HCBP12; M42C60; Mdt1; Psdr1; ralR1; SCALD; Ube-1c; UBE-1c1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204536 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGGATTCCTGCTTCTGCTCTCTCTTCCCTTCATCCTGTACTTGGTCACGCCAAAAATCAGGAAAA
  TGCTGTCCAGTGGGGTGTGCACATCTAATGTTCAGCTTCCCGGGAAGGTAGCCATAGTCACTGGTGCTAA
  CACAGGCATTGGGAAGGAGACAGCTAAAGATCTGGCCCAAAGAGGAGCCCGTGTGTATTTAGCTTGCCGG
  GATGTGGACAAGGGGGAACTGGCGGCTCGTGAGATCCAAGCCGTCACAGGGAACAGTCAGGTCTTCGTAC
  GGAAACTGGACCTAGCTGATACCAAGTCTATTCGAGCCTTTGCCAAAGACTTCTTAGCTGAGGAAAAGCA
  TCTGCACCTTCTCATCAACAATGCGGGCGTGATGATGTGCCCCTACTCGAAGACTGCAGACGGCTTTGAG
  ATGCACATTGGCGTCAACCACCTGGGTCACTTCCTCCTGACCCATTTGCTGCTAGAAAAGCTGAAGGAGT
  CGGCCCCATCAAGGATAGTCAACTTGTCTTCTTTGGGACACCATCTGGGCAGGATCCACTTCCATAACCT
  GCAGGGGGAGAAGTTCTACAGTGCGGGTCTCGCGTACTGCCACAGCAAACTAGCCAACATTCTCTTCACT
  AAGGAGCTGGCCAAGAGGCTGAAAGGTTCTGGAGTGACAACATACTCTGTACACCCTGGCACAGTCCATT
  CTGAATTGACGCGGTACTCCTCTATTATGAGATGGCTTTGGCAACTTTTCTTTGTTTTCATCAAGACCCC
  TCAAGAGGGAGCTCAGACGAGCCTGTACTGTGCCCTGACAGAAGGTCTCGAGAGCCTAAGTGGCAGTCAT
  TTCAGTGATTGCCAGTTGGCATGGGTCTCTTACCAAGGTCGCAATGAGATAATAGCCAGGCGGCTGTGGG
  ATGTCAGCTGTGACCTGCTGGGCCTCCCAGTGGATTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204536 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFGFLLLLSLPFILYLVTPKIRKMLSSGVCTSNVQLPGKVAIVTGANTGIGKETAKDLAQRGARVYLACR
  DVDKGELAAREIQAVTGNSQVFVRKLDLADTKSIRAFAKDFLAEEKHLHLLINNAGVMMCPYSKTADGFE
  MHIGVNHLGHFLLTHLLLEKLKESAPSRIVNLSSLGHHLGRIHFHNLQGEKFYSAGLAYCHSKLANILFT
  KELAKRLKGSGVTTYSVHPGTVHSELTRYSSIMRWLWQLFFVFIKTPQEGAQTSLYCALTEGLESLSGSH
  FSDCQLAWVSYQGRNEIIARRLWDVSCDLLGLPVDW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021557
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021557.2, NM_021557.3, NM_021557.4, NM_021557.5, NP_067532.2
• RefSeq Size: 1574 bp
• RefSeq ORF: 951 bp
• Locus ID: 17252
• MW: 35.1 kDa
• Gene Summary: Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent all-trans-retinal reductase. Also involved in the metabolism of short-chain aldehydes. [UniProtKB/Swiss-Prot Function]