Rnf41 (NM_026259) Mouse Tagged ORF Clone

CAT#: MR204564

Rnf41 (Myc-DDK-tagged) - Mouse ring finger protein 41 (Rnf41), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf41
• Synonyms: 2210404G21Rik; 4930511A05Rik; 4933415P08Rik; D10Ertd722e; FLRF; Nrdp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204564 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTATGATGTGACCCGTTTCCAGGGGGACGTTGACGAGGACCTTATCTGCCCTATTTGCAGTGGAG
  TCTTGGAGGAGCCAGTGCAGGCGCCTCATTGTGAGCATGCCTTCTGCAACGCCTGCATCACCCAATGGTT
  CTCTCAGCAGCAGACGTGTCCGGTAGACCGTAGCGTTGTCACGGTTGCCCACCTGCGCCCAGTACCTCGG
  ATCATGCGGAACATGTTGTCAAAGCTACAGATTGCCTGTGACAACGCTGTGTTTGGCTGCAGTGCTGTTG
  TCAGGCTTGACAACCTCATGTCCCACCTCAGTGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGA
  GCAGGGCTGTGGCCTGGAGATGCCCAAAGATGAACTGCCAAACCACAATTGCATTAAGCACCTGCGCTCC
  GTGGTCCAGCAGCAGCAGTCGCGCATTGCAGAGCTGGAGAAGACCTCGGCTGAACACAAGCACCAGCTGG
  CAGAGCAGAAGCGAGACATTCAGCTGCTGAAGGCGTATATGCGAGCCATCCGCAGTGTCAACCCCAACCT
  TCAGAACCTGGAGGAGACAATCGAATACAATGAGATCCTCGAGTGGGTGAACTCCCTGCAGCCGGCAAGG
  GTGACCCGCTGGGGGGGCATGATCTCCACTCCTGATGCTGTGCTCCAGGCTGTCATCAAGCGCTCCCTCG
  TGGAAAGTGGCTGCCCGGCCTCCATCGTCAACGAGCTGATTGAAAATGCCCATGAACGCAGCTGGCCCCA
  GGGTCTGGCCACACTAGAGACAAGACAGATGAACCGGCGCTACTATGAGAACTACGTGGCCAAGCGCATC
  CCTGGCAAGCAGGCTGTAGTGGTGATGGCCTGTGAGAACCAGCACATGGGGGACGACATGGTGCAGGAGC
  CAGGGCTCGTCATGATATTTGCGCATGGTGTGGAGGAGATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204564 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPR
  IMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRS
  VVQQQQSRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPAR
  VTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRI
  PGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026259
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026259.1, NM_026259.2, NM_026259.3, NP_080535.2
• RefSeq Size: 3341 bp
• RefSeq ORF: 954 bp
• Locus ID: 67588
• MW: 35.9 kDa