Mettl9 (NM_021554) Mouse Tagged ORF Clone

CAT#: MR204594

Mettl9 (Myc-DDK-tagged) - Mouse methyltransferase like 9 (Mettl9)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mettl9
• Synonyms: 0610012D09Rik; AA517660; Drev; MNCb-5680
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204594 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTGTTGGCGGGCTGGCTGTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAGGATGTGGACGC
  TGCGGAGCCCGCTCTCCCGCTCCCTGTACGTGAACATGACTAGCGGCCCCGGCGGGCCAGCGGCGGCCGC
  GGGCGGCGGGAAGGACACGCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTTCAGTCT
  GTCTTTGTTCAGAGTTATCTTGACCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCTGGCT
  GGCTATTTATCCAACTCTATCATTCTTTTGTATCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTAT
  TAACGGGTTGCTAGGAAGAGGCTCCATGTTTGTGTTCTCACCAGATCAGTTTCAGAGACTGCTTAGAATT
  AATCCGGACTGGAAAACCCATAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACGAAAATCATGA
  GCCCTCATTTTGAAGAAATTTACGCCACTGAGCTTTCTGAAACAATGATCTGGCAGCTCCAGAAGAAGAA
  ATACAGAGTGCTTGGTATAAATGAATGGCAGAATACAGGGTTCCAGTATGATGTCATCAGCTGCTTAAAT
  TTGCTGGATCGCTGTGATCAGCCCCTGACATTGTTAAAAGATATCAGAAGTGTCTTGGAGCCCACCCAAG
  GCAGGGTCATCCTGGCATTGGTTTTGCCCTTTCATCCCTATGTAGAAAACGTAGGTGGCAAGTGGGAGAA
  ACCATCAGAAATTCTGGAAATCAAGGGACAGAATTGGGAAGAGCAAGTGAATAGCCTGCCTGAGGTGTTC
  AGAAAAGCCGGCTTTGTCGTCGAAGCTTTCACTAGACTGCCATACCTGTGTGAAGGCGACATGTACAATG
  ACTACTATGTTCTGGACGATGCTGTCTTTGTTCTCAGACCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204594 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLLAGWLCLSLASVWLARRMWTLRSPLSRSLYVNMTSGPGGPAAAAGGGKDTHQWYVCNREKLCESLQS
  VFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLRI
  NPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLN
  LLDRCDQPLTLLKDIRSVLEPTQGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVF
  RKAGFVVEAFTRLPYLCEGDMYNDYYVLDDAVFVLRPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021554
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021554.2, NP_067529.2
• RefSeq Size: 1850 bp
• RefSeq ORF: 957 bp
• Locus ID: 59052
• MW: 36.4 kDa