H2-Q10 (NM_010391) Mouse Tagged ORF Clone

CAT#: MR204741

H2 (Myc-DDK-tagged) - Mouse histocompatibility 2, Q region locus 10 (H2-Q10)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: H2-Q10
• Synonyms: H-2Q10; H2-K1; Q10; Qa10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204741 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCGATGGCGCCGCGCACGCTGCTCCTGCTGCTGGCGGCTGCCCTGGCCCCGACCCAGACCCAGG
  CAGGCTCACACTCCATGAGGTATTTCGAAACCTCCGTTTCCCGGCCGGGCCTTGGGGAGCCCCGGTTCAT
  TATTGTCGGTTACGTGGACGACACGCAGTTCGTGCGCTTCGACAGCGACGCGGAGACTCCGAGGATGGAG
  CCGCGGGCGCCTTGGATGGAGCAGGAGGGGCCGGAGTATTGGGAGCGGGAGACACAGAGAGCCAAGGGCA
  ATGAGCAGAGTTTCCGTGTGAGCCTGAGGACCCTGCTCGGCTACTACAACCAGAGCGAGAGCGGCTCTCA
  CACGATCCAGTGGATGTATGGCTGTAAAGTGGGGTCCGACGGGCGCTTCCTCCGCGGGTACCTGCAATAC
  GCATACGACGGCCGCGATTACATTGCCCTGAACGAAGACCTGAAAACGTGGACGGCAGCGGACGTGGCGG
  CGATTATCACCCGACGCAAGTGGGAGCAGGCTGGTGCTGCAGAGTATTACAGGGCCTACCTGGAGGCCGA
  GTGCGTGGAGTGGCTCCTCAGATACCTGGAGCTCGGGAAGGAGACGCTGCTGCGCACAGATCCCCCAAAG
  ACACATGTGACCCATCACCCAGGATCTGAAGGTGATGTCACCCTGAGGTGTTGGGCCCTGGGCTTCTACC
  CTGCTGACATCACCCTGACCTGGCAGTTGAATGGGGAGGAGCTGACCCAGGACATGGAACTGGTGGAGAC
  CAGGCCTGCAGGGGATGGAACCTTCCAGAAGTGGGCATCTGTGGTGGTGCCTCTTGGGAAGGAGCAGAAT
  TACACATGCCATGTGTACCATGAGGGGCTGCCTGAGCCCCTCACCCTGAGATGGGAACCTCCTCCTTCCA
  CTGACTCTATCATGTCACACATTGCTGATCTGCTGTGGCCATCATTAAAGCTCTGGTGGTATTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204741 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAMAPRTLLLLLAAALAPTQTQAGSHSMRYFETSVSRPGLGEPRFIIVGYVDDTQFVRFDSDAETPRME
  PRAPWMEQEGPEYWERETQRAKGNEQSFRVSLRTLLGYYNQSESGSHTIQWMYGCKVGSDGRFLRGYLQY
  AYDGRDYIALNEDLKTWTAADVAAIITRRKWEQAGAAEYYRAYLEAECVEWLLRYLELGKETLLRTDPPK
  THVTHHPGSEGDVTLRCWALGFYPADITLTWQLNGEELTQDMELVETRPAGDGTFQKWASVVVPLGKEQN
  YTCHVYHEGLPEPLTLRWEPPPSTDSIMSHIADLLWPSLKLWWYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010391
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010391.1, NM_010391.2, NM_010391.3, NM_010391.4, NP_034521.1
• RefSeq Size: 1473 bp
• RefSeq ORF: 978 bp
• Locus ID: 15007
• MW: 37.3 kDa
• Gene Summary: Involved in the presentation of foreign antigens to the immune system. [UniProtKB/Swiss-Prot Function]