Aip (NM_016666) Mouse Tagged ORF Clone

CAT#: MR204848

Aip (Myc-DDK-tagged) - Mouse aryl-hydrocarbon receptor-interacting protein (Aip)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aip
• Synonyms: AA408703; Ara9; AW476050; D19Bwg1412e; Fkbp16; Xap2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204848 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATCTCATCGCAAGACTTCGAGAGGACGGGATCCAAAAGCGTGTGATACAGGAGGGCCGAGGAG
  AGTTGCCGGACTTTCAGGATGGCACTAAGGCCACGTTCCACTTTCGGACGCTGCACAGTGACAATGAAGG
  CTCCGTTATAGATGACAGCCGAACACGAGGCAAGCCCATGGAGCTCATCGTTGGCAAGAAGTTCAAGTTG
  CCTGTGTGGGAGACCATCGTGTGCACCATGCGTGAGGGGGAGATTGCCCAGTTCCTCTGTGACATCAAGC
  ACGTAGTCCTGTATCCTCTAGTGGCCAAGAGCCTCCGCAACATCGCCGAGGGCAAGGACCCCCTGGAGGG
  ACAGCGGCATTGCTGTGGGATCGCTCAGATGCATGAACACAGCTCCCTGGGTCATGCGGACCTGGATGCC
  CTGCAGCAGAACCCACAGCCTCTCATCTTCCACATCGAGATGCTGAAGGTAGAGAGTCCTGGCACCTATC
  AACAGGACCCATGGGCGATGACAGATGAGGAGAAAGCAAAGGCAGTACCAGTCATCCACCAAGAGGGCAA
  CCGGTTGTACCGTGAGGGCCAGGTGAAAGAGGCTGCTGCCAAGTACTATGACGCCATCGCCTGCCTCAAG
  AATCTGCAGATGAAGGAGCAGCCTGGCTCACCTGACTGGATCCAGTTGGACCTGCAGATCACACCACTGC
  TACTCAACTATTGCCAGTGCAAGCTGGTGGCTCAGGAGTATTATGAAGTGTTGGATCACTGCTCCTCCAT
  CCTCAACAAGTATGATGACAATGTCAAGGCTTACTTCAAGAGGGGTAAGGCCCATGCTGCCGTGTGGAAT
  GCCCAGGAGGCCCAGGCTGACTTTGCCAAGGTGTTGGAGCTAGACCCTGCCCTGGCGCCTGTGGTAAGCA
  GAGAGCTGCGGGCCCTGGAGACACGGATCCGGCAGAAGGATGAGGAGGACAAAGCTCGCTTCCGGGGCAT
  CTTTTCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204848 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADLIARLREDGIQKRVIQEGRGELPDFQDGTKATFHFRTLHSDNEGSVIDDSRTRGKPMELIVGKKFKL
  PVWETIVCTMREGEIAQFLCDIKHVVLYPLVAKSLRNIAEGKDPLEGQRHCCGIAQMHEHSSLGHADLDA
  LQQNPQPLIFHIEMLKVESPGTYQQDPWAMTDEEKAKAVPVIHQEGNRLYREGQVKEAAAKYYDAIACLK
  NLQMKEQPGSPDWIQLDLQITPLLLNYCQCKLVAQEYYEVLDHCSSILNKYDDNVKAYFKRGKAHAAVWN
  AQEAQADFAKVLELDPALAPVVSRELRALETRIRQKDEEDKARFRGIFSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016666
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016666.1, NM_016666.2, NM_016666.3, NP_057875.1
• RefSeq Size: 2068 bp
• RefSeq ORF: 993 bp
• Locus ID: 11632
• MW: 37.6 kDa
• Gene Summary: This gene encodes an Hsp90-associated protein that is localized to the cytoplasm. The encoded protein interacts with the aryl hydrocarbon receptor (AhR) and the tyrosine kinase receptor RET, inhibits AhR ubiquitination, and regulates the intracellular localization of AhR. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]