Acot7 (NM_133348) Mouse Tagged ORF Clone

CAT#: MR205033

Acot7 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 7 (Acot7), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acot7
• Synonyms: 2410041A17Rik; Ach1; Act; Bach; Cte-II; CTE-IIa; Lach1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205033 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCCCCACCACAGACACGCCGGCCGCCATCCAGATCTGCCGGATCATGCGTCCAGATGATGCCA
  ATGTGGCTGGCAATGTTCATGGAGGGACCATTCTGAAGATGATTGAGGAGGCCGGGGCCATCATCAGCAC
  GCGGCACTGTAACAGCCAGAATGGGGAGCGCTGTGTGGCTGCCCTGGCACGGGTGGAGCGCACTGACTTC
  CTGTCACCCATGTGCATCGGCGAGGTGGCTCATGTCAGTGCAGAGATCACCTACACTTCCAAGCACTCTG
  TGGAGGTCCAGGTCCACGTGATGTCGGAGAACATCCTCACAGGTACCAAAAAGCTGACCAATAAAGCCAC
  CTTGTGGTATGTGCCCCTGTCATTGAAGAATGTGGACAAGGTCCTTGAGGTGCCTCCCATTGTGTATTTA
  CGGCAGGAGCAGGAGGAGGAGGGTCGGAAACGCTATGAAGCCCAGAAGCTGGAACGCATGGAGACCAAGT
  GGAGGAACGGAGACATTGTCCAGCCTGTCCTGAACCCAGAGCCGAACACGGTGAGCTACAGCCAGTCCAG
  CCTGATCCACCTGGTGGGGCCCTCGGACTGCACCCTTCATGGCTTCGTGCACGGAGGTGTCACCATGAAG
  CTCATGGATGAGGTGGCTGGGATTGTGGCTGCACGCCACTGCAAGACCAACATAGTAACTGCCTCTGTGG
  ATGCCATCAATTTCCACGACAAGATCCGGAAAGGCTGTGTCATCACCATCTCCGGACGCATGACCTTCAC
  AAGCAATAAGTCCATGGAGATTGAGGTCCTGGTGGACGCTGACCCTGTGGTGGACAACTCACAAAAGCGC
  TACCGGGCCGCCAGTGCCTTCTTCACCTACGTGTCCCTGAACCAGGAGGGCAAGCCAATGCCTGTGCCTC
  AGCTTGTGCCAGAGACGGAGGATGAGAAGAAGCGCTTCGAAGAAGGCAAAGGCCGTTATCTGCAGATGAA
  GGCGAAGCGACAGGGCCACACAGAGCCTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205033 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGPTTDTPAAIQICRIMRPDDANVAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDF
  LSPMCIGEVAHVSAEITYTSKHSVEVQVHVMSENILTGTKKLTNKATLWYVPLSLKNVDKVLEVPPIVYL
  RQEQEEEGRKRYEAQKLERMETKWRNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMK
  LMDEVAGIVAARHCKTNIVTASVDAINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDNSQKR
  YRAASAFFTYVSLNQEGKPMPVPQLVPETEDEKKRFEEGKGRYLQMKAKRQGHTEPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133348
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133348.1, NP_579926.1
• RefSeq Size: 1494 bp
• RefSeq ORF: 1140 bp
• Locus ID: 70025
• MW: 37.6 kDa