Aurkb (NM_011496) Mouse Tagged ORF Clone

CAT#: MR205201

Aurkb (Myc-DDK-tagged) - Mouse aurora kinase B (Aurkb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aurkb
• Synonyms: Aik2; AIM-1; Aim1; AIRK2; AL022959; Ark2; AurB; IPL1; STK-1; Stk5; Stk12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205201 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGAAGGAGAACGCCTACCCGTGGCCCTACGGCTCAAAGACGTCTCAGTCTGGCCTGAACACGT
  TGTCCCAGAGAGTCCTACGGAAGGAGCCCGCCACGACATCTGCGCTTGCTCTCGTGAACCGGTCCAACAG
  CCAGTCCACAGCTGCCCCTGGCCAGAAGTTGGCTGAGAACAAGAGTCAGGGCTCCACTGCCTCGCAAGGA
  TCCCAGAACAAGCAGCCTTTCACTATTGACAACTTTGAGATTGGGCGTCCTTTGGGCAAAGGCAAATTTG
  GAAACGTGTACTTGGCTCGGGAGAAGAAGAGCCGTTTCATCGTGGCACTCAAGATCCTCTTCAAGTCTCA
  GATTGAGAAGGAGGGGGTAGAGCACCAGCTTCGCCGAGAGATCGAAATCCAGGCGCACCTGAAACATCCC
  AACATCCTTCAACTCTACAACTACTTCTACGACCAGCAGAGGATCTACTTAATCCTGGAATACGCCCCTC
  GCGGGGAACTCTACAAGGAACTGCAGAAGAGTCGGACCTTCGATGAGCAGCGGACTGCCACGATCATGGA
  GGAACTGTCAGATGCCCTGACCTACTGCCACAAGAAGAAGGTAATTCACAGAGACATAAAGCCGGAGAAC
  CTGCTGTTAGGTCTGCAGGGAGAACTGAAGATTGCAGACTTTGGCTGGTCGGTGCATGCCCCATCCCTGA
  GGAGGAAGACCATGTGCGGCACGCTGGACTATCTGCCCCCAGAGATGATTGAGGGGCGCATGCATAATGA
  AATGGTAGATCTATGGTGCATCGGGGTGCTCTGCTATGAACTGATGGTGGGGAACCCACCCTTCGAGAGC
  CCTAGCCACAGTGAGACGTATCGTCGGATTGTCAAGGTGGACCTGAAGTTCCCCTCTTCTGTGCCTTCGG
  GCGCCCAGGACCTCATCTCCAAGCTGCTCAAACATAACCCCTGGCAACGGCTGCCCCTGGCGGAGGTTGC
  AGCTCACCCTTGGGTCCGGGCCAACTCAAGGAGGGTTCTGCCTCCCTCTGCCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205201 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQKENAYPWPYGSKTSQSGLNTLSQRVLRKEPATTSALALVNRSNSQSTAAPGQKLAENKSQGSTASQG
  SQNKQPFTIDNFEIGRPLGKGKFGNVYLAREKKSRFIVALKILFKSQIEKEGVEHQLRREIEIQAHLKHP
  NILQLYNYFYDQQRIYLILEYAPRGELYKELQKSRTFDEQRTATIMEELSDALTYCHKKKVIHRDIKPEN
  LLLGLQGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEMVDLWCIGVLCYELMVGNPPFES
  PSHSETYRRIVKVDLKFPSSVPSGAQDLISKLLKHNPWQRLPLAEVAAHPWVRANSRRVLPPSAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011496
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011496.1, NM_011496.2, NP_035626.1
• RefSeq Size: 1957 bp
• RefSeq ORF: 1038 bp
• Locus ID: 20877
• MW: 39.3 kDa
• Gene Summary: This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 2 and 7. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. [provided by RefSeq, Sep 2015]