Efnb1 (NM_010110) Mouse Tagged ORF Clone

CAT#: MR205205

Efnb1 (Myc-DDK-tagged) - Mouse ephrin B1 (Efnb1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Efnb1
• Synonyms: Cek5-L; EFL-3; Elk-L; Epl2; Eplg2; LERK-2; Lerk2; Stra1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205205 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGCCTGGGCAGCGTTGGCTCAGCAAGTGGCTTGTGGCTATGGTCGTGCTGACGCTGTGCCGGC
  TTGCCACGCCGTTGGCCAAGAACCTGGAGCCCGTGTCCTGGAGCTCTCTTAACCCTAAGTTCCTAAGTGG
  GAAGGGCTTGGTGATCTACCCGAAGATTGGAGACAAGCTGGACATCATCTGCCCCCGAGCAGAAGCAGGG
  CGGCCCTACGAGTACTACAAGCTGTACCTGGTGCGGCCAGAGCAGGCGGCTGCTTGCAGCACTGTGCTTG
  ATCCCAATGTACTGGTCACTTGCAACAAGCCACACCAGGAAATCCGCTTCACCATCAAGTTCCAAGAGTT
  CAGCCCCAACTACATGGGCCTGGAATTCAAAAAGTACCACGATTACTACATTACATCAACGTCCAATGGG
  AGCTTGGAGGGACTGGAGAACCGGGAGGGAGGTGTGTGTCGCACCCGCACTATGAAGATCGTTATGAAGG
  TTGGGCAAGATCCAAATGCTGTGACACCCGAGCAGTTGACTACCAGCCGGCCAAGCAAGGAGTCAGACAA
  CACTGTCAAGACAGCCACACAGGCTCCTGGTCGGGGATCCCAGGGTGACTCTGACGGCAAGCATGAGACT
  GTGAACCAGGAAGAGAAGAGTGGCCCAGGTGCAGGTGGCGGTGGCAGCGGGGACTCTGACAGCTTCTTCA
  ACTCCAAGGTAGCATTGTTCGCAGCCGTCGGCGCCGGCTGTGTCATCTTCCTGCTCATCATCATCTTCTT
  GACAGTCCTACTACTCAAGCTCCGCAAGCGCCATCGCAAGCATACACAGCAGCGGGCGGCTGCCCTCTCG
  CTCAGTACCCTAGCCAGCCCCAAAGGGGGTAGTGGTACAGCGGGCACCGAGCCCAGCGACATCATCATCC
  CCTTACGGACTACAGAGAACAACTACTGCCCCCACTATGAGAAGGTGAGTGGGGACTACGGGCATCCTGT
  CTACATCGTCCAGGAGATGCCCCCTCAGAGCCCGGCGAACATCTACTACAAGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205205 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARPGQRWLSKWLVAMVVLTLCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAG
  RPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNKPHQEIRFTIKFQEFSPNYMGLEFKKYHDYYITSTSNG
  SLEGLENREGGVCRTRTMKIVMKVGQDPNAVTPEQLTTSRPSKESDNTVKTATQAPGRGSQGDSDGKHET
  VNQEEKSGPGAGGGGSGDSDSFFNSKVALFAAVGAGCVIFLLIIIFLTVLLLKLRKRHRKHTQQRAAALS
  LSTLASPKGGSGTAGTEPSDIIIPLRTTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010110
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010110.1, NM_010110.2, NM_010110.3, NM_010110.4, NM_010110.5, NP_034240.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 1038 bp
• Locus ID: 13641
• MW: 37.9 kDa
• Gene Summary: This gene encodes a membrane protein that acts as a ligand for Eph family receptors. Signalling occurs bidirectionally in both the cell containing the receptor and the cell expressing this protein. Activity of this protein is important in neuronal axon growth and other developmental processes. [provided by RefSeq, May 2015]