Yipf3 (NM_145353) Mouse Tagged ORF Clone

CAT#: MR205231

Yipf3 (Myc-DDK-tagged) - Mouse Yip1 domain family, member 3 (Yipf3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Yipf3
• Synonyms: C6orf109; D17Wsu94e; KLIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205231 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTCCGGCGGCGCCGGCCAGCGGCGTCCGAAATGGTGCTGGCCCGGAATGGGGAGGCTTCGAAG
  AAAACATCCAGGGTGGGGGTTCGGCTGTGATTGATATGGAGAACATGGACGATACCTCAGGCTCCAGCTT
  CGAGGACATGGGTGAGCTGCATCAGCGCCTGCGGGAGGAAGAAGTAGATGCTGATGCAGCTGCTGCAGAA
  GAAGAGGATGGGGAGTTTCTTGGCATGAAAGGCTTTAAGGGACAACTGAGCCGGCAGGTAGCAGATCAGA
  TGTGGCAGGCAGGGAAGAGACAGGCTTCCAGGGCCTTCAGCTTGTATGCCAACATTGACATCCTCAGACC
  CTACTTTGATGTGGAGCCTGCCCAGGTCCGAAGCAGGCTCCTGGAGTCCATGATCCCTATCAAGATGGTC
  AACTTCCCCCAGAAAGTCGCGGGCGAGCTCTACGGACCGCTCATGCTGGTCTTCACACTGGTGGCCATCC
  TCCTGCATGGAATGAAGACTTCTGACACCATTATCCGGGAGGGCACCCTCATGGGCACAGCCATAGGCAC
  CTGCTTTGGATACTGGCTGGGTGTCTCGTCCTTCATTTATTTCCTTGCCTACCTGTGTAACGCCCAGATC
  ACCATGCTACAGATGTTGGCACTGCTGGGCTACGGCCTCTTCGGGCACTGCATAGTCCTGTTCATCACCT
  ATAACATCCACCTTCATGCCCTCTTCTACCTCTTCTGGCTGCTGGTGGGTGGGCTCTCTACCCTGCGCAT
  GGTGGCAGTGTTGGTGTCGCGGACTGTGGGCCCTACGCAGCGGCTGCTGCTCTGTGGCACGCTGGCTGCC
  CTGCACATGCTCTTCCTGCTCTATCTGCACTTCGCCTACCACAAAGTGGTGGAGGGGATCCTGGACACCC
  TGGAGGGCCCCAACATCCCACCCATGCAGAGGGTCCCCAGAGACATCCCCGCTGTGCTCCCTGCTGCCAG
  GCTGCCCGTCGCTGTGGTCAATGCTACCGCCAAGGCAATAGCAGTGACTCTGCAGTCACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205231 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATPAAPASGVRNGAGPEWGGFEENIQGGGSAVIDMENMDDTSGSSFEDMGELHQRLREEEVDADAAAAE
  EEDGEFLGMKGFKGQLSRQVADQMWQAGKRQASRAFSLYANIDILRPYFDVEPAQVRSRLLESMIPIKMV
  NFPQKVAGELYGPLMLVFTLVAILLHGMKTSDTIIREGTLMGTAIGTCFGYWLGVSSFIYFLAYLCNAQI
  TMLQMLALLGYGLFGHCIVLFITYNIHLHALFYLFWLLVGGLSTLRMVAVLVSRTVGPTQRLLLCGTLAA
  LHMLFLLYLHFAYHKVVEGILDTLEGPNIPPMQRVPRDIPAVLPAARLPVAVVNATAKAIAVTLQSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145353
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145353.1, NM_145353.2, NP_663328.1
• RefSeq Size: 2035 bp
• RefSeq ORF: 1044 bp
• Locus ID: 28064
• MW: 38 kDa