Bag1 (NM_001171739) Mouse Tagged ORF Clone

CAT#: MR205418

Bag1 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 1 (Bag1), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bag1
• Synonyms: BAG-1; Rap46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205418 representing NM_001171739
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGCGGGTCGCAGCGCCGCGCGGCGACCGCGAGGCGACCGGGAGCCGCTGGGACCCAGGCTGCGCGCCC
  CTCGGCCTGCCCGGGAGCCGCGCCAGTCGGAGTCCCGGGCCGAGCGGGGCTTGCCTCCCTCTCAGCGTTC
  CTCTGTGCGCAGTGCAGCCAGCGGGCATGACCGATCCACCAGGGGCGCCGCCGCCGGCGCCTGCAAGCCG
  CGGGTGAAGAAGAAAGTCCGGCCCCGCTCTTCTCAGAGCGAGAAGGTAGGGAGCAGCAGCAGGGAATTGA
  CTAGAAGTAAGAAAGTGACCCGTAGCAAGAACGTGACCGGGACCCAGGTAGAGGAGGTGACCAAGATCGA
  GGAGGCGACCCAAACCGAGGAAGTAACTGTGGCAGAAGAGGTGACCCAGACCGACAACATGGCCAAGACC
  GAGGAGATGGTCCAGACGGAGGAAATGGAAACACCCAGACTCAGCGTGATCGTCACCCACAGCAATGAGA
  GGTATGACCTTCTTGTTACCCCACAGCAAGGTAACAGTGAGCCAGTTGTCCAAGACTTGGCTCAGCTTGT
  TGAAGAGGCCACAGGAGTTCCACTACCTTTTCAGAAGCTCATATTTAAGGGAAAATCTCTGAAAGAAATG
  GAAACACCGTTGTCAGCACTTGGAATGCAAAATGGTTGCCGAGTCATGTTAATTGGTGAAAAGAGCAATC
  CAGAAGAAGAGGTTGAGTTAAAGAAGCTGAAAGATTTGGAGGTATCTGCAGAGAAGATAGCTAACCACCT
  GCAAGAATTGAATAAAGAGCTTTCTGGCATCCAGCAGGGTTTTCTGGCTAAGGAATTGCAAGCGGAGGCT
  CTCTGCAAACTTGATAGGAAAGTAAAAGCAACAATTGAGCAATTCATGAAGATCTTGGAGGAGATTGACA
  CAATGGTCCTACCAGAACAATTTAAAGACAGCAGGCTAAAAAGGAAGAATTTGGTGAAAAAGGTTCAGGT
  GTTCTTAGCAGAGTGTGACACAGTGGAGCAATACATCTGCCAAGAGACAGAGCGGCTGCAGTCTACAAAC
  TTGGCCCTGGCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205418 representing NM_001171739
  Red=Cloning site Green=Tags(s)
  
  LAGRSAARRPRGDREPLGPRLRAPRPAREPRQSESRAERGLPPSQRSSVRSAASGHDRSTRGAAAGACKP
  RVKKKVRPRSSQSEKVGSSSRELTRSKKVTRSKNVTGTQVEEVTKIEEATQTEEVTVAEEVTQTDNMAKT
  EEMVQTEEMETPRLSVIVTHSNERYDLLVTPQQGNSEPVVQDLAQLVEEATGVPLPFQKLIFKGKSLKEM
  ETPLSALGMQNGCRVMLIGEKSNPEEEVELKKLKDLEVSAEKIANHLQELNKELSGIQQGFLAKELQAEA
  LCKLDRKVKATIEQFMKILEEIDTMVLPEQFKDSRLKRKNLVKKVQVFLAECDTVEQYICQETERLQSTN
  LALAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171739
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq Size: 1365
• RefSeq ORF: 660
• Locus ID: 12017
• MW: 25.3 kDa
• Gene Summary: The oncogene Bcl2 encodes a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to Bcl2 protein and is referred to as Bcl2-associated athanogene. It enhances the anti-apoptotic effects of Bcl2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least two protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site and an alternative, downstream, AUG translation initiation site. [provided by RefSeq, Jul 2008]