Vegfd (NM_010216) Mouse Tagged ORF Clone

CAT#: MR205479

Figf (Myc-DDK-tagged) - Mouse c-fos induced growth factor (Figf)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vegfd
• Synonyms: AI325264; Figf; VEGF-D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205479 representing NM_010216
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGGAGAATGGGGAATGGGGAATATCCTCATGATGTTCCATGTGTACTTGGTGCAGGGCTTCAGGA
  GCGAACATGGACCAGTGAAGGATTTTTCTTTTGAGCGATCATCCCGGTCCATGTTGGAACGATCTGAACA
  ACAGATCCGAGCAGCTTCTAGTTTGGAGGAGTTGCTGCAAATCGCGCACTCTGAGGACTGGAAGCTGTGG
  CGATGCCGGTTGAAGCTCAAAAGTCTTGCCAGTATGGACTCACGCTCAGCATCCCATCGCTCCACCAGAT
  TTGCGGCAACTTTCTATGACACTGAAACACTAAAAGTTATAGATGAAGAATGGCAGAGGACCCAATGCAG
  CCCTAGAGAGACATGCGTAGAAGTCGCCAGTGAGCTGGGGAAGACAACCAACACATTCTTCAAGCCCCCC
  TGTGTAAATGTCTTCCGGTGTGGAGGCTGCTGCAACGAAGAGGGTGTGATGTGTATGAACACAAGCACCT
  CCTACATCTCCAAACAGCTCTTTGAGATATCAGTGCCTCTGACATCAGTGCCCGAGTTAGTGCCTGTTAA
  AATTGCCAACCATACGGGTTGTAAGTGCTTGCCCACGGGCCCCCGCCATCCTTACTCAATTATCAGAAGA
  TCCATTCAGACCCCAGAAGAAGATGAATGTCCTCATTCCAAGAAACTCTGTCCTATTGACATGCTGTGGG
  ATAACACCAAATGTAAATGTGTTTTGCAAGACGAGACTCCACTGCCTGGGACAGAAGACCACTCTTACCT
  CCAGGAACCCACTCTCTGTGGACCGCACATGACGTTTGATGAAGATCGCTGTGAGTGCGTCTGTAAAGCA
  CCATGTCCGGGAGATCTCATTCAGCACCCGGAAAACTGCAGTTGCTTTGAGTGCAAAGAAAGTCTGGAGA
  GCTGCTGCCAAAAGCACAAGATTTTTCACCCAGACACCTGCAGCTGTGAGGACAGATGTCCTTTTCACAC
  CAGAACATGTGCAAGTAGAAAGCCAGCCTGTGGAAAGCACTGGCGCTTTCCAAAGGAGACAAGGGCCCAG
  GGACTCTACAGCCAGGAGAACCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205479 representing NM_010216
  Red=Cloning site Green=Tags(s)
  
  MYGEWGMGNILMMFHVYLVQGFRSEHGPVKDFSFERSSRSMLERSEQQIRAASSLEELLQIAHSEDWKLW
  RCRLKLKSLASMDSRSASHRSTRFAATFYDTETLKVIDEEWQRTQCSPRETCVEVASELGKTTNTFFKPP
  CVNVFRCGGCCNEEGVMCMNTSTSYISKQLFEISVPLTSVPELVPVKIANHTGCKCLPTGPRHPYSIIRR
  SIQTPEEDECPHSKKLCPIDMLWDNTKCKCVLQDETPLPGTEDHSYLQEPTLCGPHMTFDEDRCECVCKA
  PCPGDLIQHPENCSCFECKESLESCCQKHKIFHPDTCSCEDRCPFHTRTCASRKPACGKHWRFPKETRAQ
  GLYSQENP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010216
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010216.1, NM_010216.2, NP_034346.1
• RefSeq Size: 1890 bp
• RefSeq ORF: 1077 bp
• Locus ID: 14205
• MW: 41.4 kDa
• Gene Summary: This gene encodes a member of the vascular endothelial growth factor (VEGF) family that acts as a ligand for vascular endothelial growth factor receptor 3. Signalling through this protein is important for growth and development of endothelial and vascular tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]