Pdhb (NM_024221) Mouse Tagged ORF Clone

CAT#: MR205484

Pdhb (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase (lipoamide) beta (Pdhb), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdhb
• Synonyms: 2610103L06Rik; AL024199; C81408
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGGTGGCTGGTCTGGTGCGGGGACCCTTGCGGCAGGCTTCCGGGCTGCTGAAGAGGCGTTTTC
  ACCGCTCGGCGCCCGCAGCGGTGCAGTTGACAGTTCGTGAAGCTATTAATCAAGGTATGGATGAAGAACT
  AGAAAGAGATGAAAAAGTTTTTCTGCTTGGGGAAGAAGTTGCCCAGTATGACGGTGCATACAAGGTTAGC
  AGAGGCCTGTGGAAGAAATACGGTGACAAGAGGATCATCGACACCCCCATCTCAGAGATGGGCTTTGCTG
  GAATTGCTGTTGGTGCAGCTATGGCTGGGTTGCGGCCTATCTGTGAATTTATGACCTTCAATTTCTCTAT
  GCAAGCTATTGACCAGGTTATAAACTCAGCTGCCAAGACTTACTATATGTCCGCTGGCCTTCAGCCTGTG
  CCCATAGTATTCAGGGGGCCCAATGGTGCCTCAGCAGGTGTAGCTGCTCAGCACTCGCAATGCTTTGCTG
  CATGGTATGGGCACTGCCCAGGTTTAAAAGTGGTCAGCCCCTGGAATTCCGAGGATGCAAAAGGACTTAT
  TAAATCGGCCATTCGTGATAATAACCCAGTGGTAATGCTAGAAAATGAACTGATGTATGGAGTTGCATTT
  GAACTTCCTGCAGAAGCTCAGTCAAAAGATTTCCTGATTCCTATTGGAAAAGCCAAAATTGAAAGGCAAG
  GGACCCACATCACTGTAGTTGCCCATTCAAGACCAGTGGGCCACTGCCTAGAAGCTGCAGCTGTATTGTC
  TAAGGAGGGAATTGAATGTGAGGTAATAAATCTGCGCACCATCAGACCAATGGACATCGAAGCCATAGAA
  GCCAGTGTGATGAAGACAAACCATCTCGTGACTGTGGAAGGAGGCTGGCCACAATTTGGAGTAGGAGCTG
  AGATTTGTGCCAGAATTATGGAAGGCCCTGCATTCAACTTCCTTGATGCCCCTGCCGTGCGTGTCACTGG
  TGCTGATGTCCCTATGCCTTACGCAAAGGTCCTAGAAGACAACTCCGTACCTCAGGTCAAAGACATCATA
  TTTGCAGTAAAGAAGACTCTGAACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMDEELERDEKVFLLGEEVAQYDGAYKVS
  RGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPV
  PIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAF
  ELPAEAQSKDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIE
  ASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDII
  FAVKKTLNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024221
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024221.1, NM_024221.2, NM_024221.3, NP_077183.1
• RefSeq Size: 1485
• RefSeq ORF: 1080
• Locus ID: 68263
• MW: 38.9 kDa