Gna11 (NM_010301) Mouse Tagged ORF Clone

CAT#: MR205495

Gna11 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha 11 (Gna11)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gna11
• Synonyms: Dsk7; E430025L19; g alpha-11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205495 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTGGAGAACTTGATGGCGTGTTGCCTGAGCGACGAGGTGAAGGAGTCGAAGCGCATCAACGCGG
  AGATCGAGAAACAGCTGCGGCGGGACAAGCGCGACGCCCGGCGCGAGCTCAAGCTGCTGCTACTTGGCAC
  TGGCGAGAGCGGGAAGAGTACCTTCATCAAGCAGATGCGCATCATCCACGGGGCCGGCTACTCGGAGGAG
  GACAAGCGCGGCTTCACCAAGTTGGTGTACCAGAACATCTTTACCGCCATGCAGGCCATGGTGCGCGCCA
  TGGCGACGCTCAAGATCCTCTACAAGTATGAGCAGAACAAGGCCAATGCACTCCTGATCCGGGAGGTCGA
  CTTGGAGAAGGTCACAACTTTTGAGCACCAGTATGTGAATGCCATCAAGACGCTGTGGAGTGACCCTGGT
  GTCCAGGAGTGTTACGATCGCAGGCCTGAGTTCCAGCTATCTGACTCGGCTAAGTACTACTTGACGGACG
  TGGACCGCATCGCCACAGTAGGCTACCTGCCCACCCAGCAGGATGTGCTGCGGGTACGCGTGCCCACCAC
  TGGCATCATCGAGTACCCGTTTGACCTGGAGAACATCATCTTCAGGATGGTGGATGTGGGCGGGCAGAGG
  TCAGAGCGCAGGAAGTGGATCCATTGCTTTGAGAACGTGACTTCCATCATGTTCTTGGTGGCACTAAGCG
  AGTATGACCAAGTCCTGGTGGAGTCAGACAATGAGAACCGCATGGAGGAGAGCAAGGCCCTGTTCCGCAC
  AATCATCACCTACCCCTGGTTCCAGAACTCGTCTGTCATTCTCTTCCTCAACAAGAAGGACCTTCTAGAA
  GACAAGATCCTGCACTCACACTTGGTCGATTACTTCCCTGAGTTTGATGGGCCACAGAGGGATGCACAGG
  CCGCACGCGAGTTCATTCTGAAGATGTTTGTGGACCTGAACCCTGACAGCGACAAAATCATCTACTCCCA
  CTTCACGTGTGCCACCGACACCGAGAACATCCGCTTTGTGTTCGCAGCTGTGAAGGACACCATCCTGCAG
  CTGAACCTGAAGGAGTACAACCTGGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205495 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTLENLMACCLSDEVKESKRINAEIEKQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGAGYSEE
  DKRGFTKLVYQNIFTAMQAMVRAMATLKILYKYEQNKANALLIREVDLEKVTTFEHQYVNAIKTLWSDPG
  VQECYDRRPEFQLSDSAKYYLTDVDRIATVGYLPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQR
  SERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLE
  DKILHSHLVDYFPEFDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQ
  LNLKEYNLV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010301
• ORF Size: 1080 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010301.1, NM_010301.2, NM_010301.3, NP_034431.1
• RefSeq Size: 3262 bp
• RefSeq ORF: 1080 bp
• Locus ID: 14672
• MW: 41.9 kDa
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C. [UniProtKB/Swiss-Prot Function]