Ptgfr (NM_008966) Mouse Tagged ORF Clone

CAT#: MR205626

Ptgfr (Myc-DDK-tagged) - Mouse prostaglandin F receptor (Ptgfr)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptgfr
• Synonyms: AI957154; fp; PGF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205626 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATGAACAGTTCCAAGCAGCCAGTGTCTCCTGCAGCTGGACTCATCGCAAACACAACCTGCCAGA
  CGGAGAACCGGCTTTCAGTATTTTTTTCTATCATCTTCATGACAGTGGGGATCTTATCGAACAGCCTGGC
  CATCGCCATTCTCATGAAGGCCTACCAGAGATTTAGACAGAAGTCAAAGGCTTCCTTCCTGCTTTTGGCT
  AGTGGCCTGGTGATCACAGACTTCTTTGGCCACCTTATCAACGGAGGCATAGCTGTCTTTGTATATGCTT
  CTGATAAAGACTGGATCCGCTTTGATCAGTCAAACATCCTGTGCAGTATTTTTGGAATCTCCATGGTCTT
  TTCTGGCTTGTGCCCACTTTTTCTAGGCAGTGCGATGGCCATCGAGAGGTGTATAGGAGTCACCAATCCT
  ATATTTCACTCTACGAAGATCACATCTAAACACGTGAAAATGATCCTGAGTGGTGTGTGCATGTTTGCTG
  TGTTCGTGGCTGTGCTGCCCATCCTTGGACACCGAGATTATCAAATCCAAGCGTCCAGAACCTGGTGTTT
  CTACAACACAGAGCACATCGAAGACTGGGAAGACAGATTTTATCTCCTGTTCTTTTCTTTCCTCGGACTC
  TTAGCTCTTGGTGTTTCCTTCTCGTGCAATGCCGTCACGGGAGTCACACTCTTAAGAGTGAAGTTCAGAA
  GCCAGCAGCATAGGCAAGGCAGATCTCACCACCTGGAGATGATCATTCAGCTCCTGGCCATAATGTGCGT
  CTCCTGCGTCTGCTGGAGTCCCTTTCTGGTAACAATGGCCAACATTGCAATAAATGGAAATAATTCCCCA
  GTGACCTGTGAAACGACACTTTTTGCTCTCCGCATGGCAACGTGGAATCAGATCTTAGATCCCTGGGTCT
  ATATTCTGCTACGGAAGGCTGTCCTTAGGAACCTGTATAAACTTGCCAGTCGTTGCTGTGGAGTTAACAT
  CATCAGCTTGCATATCTGGGAACTCAGCTCCATCAAGAATTCCTTAAAGGTTGCTGCTATTTCTGAGTCA
  CCAGCTGCAGAGAAGGAGAGCCAGCAAGCATCGAGTGAAGCTGGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205626 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSMNSSKQPVSPAAGLIANTTCQTENRLSVFFSIIFMTVGILSNSLAIAILMKAYQRFRQKSKASFLLLA
  SGLVITDFFGHLINGGIAVFVYASDKDWIRFDQSNILCSIFGISMVFSGLCPLFLGSAMAIERCIGVTNP
  IFHSTKITSKHVKMILSGVCMFAVFVAVLPILGHRDYQIQASRTWCFYNTEHIEDWEDRFYLLFFSFLGL
  LALGVSFSCNAVTGVTLLRVKFRSQQHRQGRSHHLEMIIQLLAIMCVSCVCWSPFLVTMANIAINGNNSP
  VTCETTLFALRMATWNQILDPWVYILLRKAVLRNLYKLASRCCGVNIISLHIWELSSIKNSLKVAAISES
  PAAEKESQQASSEAGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008966
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008966.1, NM_008966.2, NM_008966.3, NP_032992.1
• RefSeq Size: 4414 bp
• RefSeq ORF: 1101 bp
• Locus ID: 19220
• MW: 40.7 kDa