Mapk12 (NM_013871) Mouse Tagged ORF Clone

CAT#: MR205654

Mapk12 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 12 (Mapk12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapk12
• Synonyms: AW123708; Erk6; P38gamma; Prkm12; Sapk3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205654 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCCGCCACCCGCCCGCAAGGGCTTTTACCGCCAGGAGGTGACCAAAACGGCCTGGGAGGTGC
  GCGCCGTGTACCAAGACCTGCAGCCCGTTGGCTCTGGTGCCTATGGTGCAGTGTGCTCTGCAGTAGACAG
  CCGCACTGGCAACAAGGTGGCCATCAAGAAGTTGTACCGGCCCTTCCAGTCGGAGCTGTTTGCCAAGCGC
  GCCTACAGAGAGTTGCGCCTCCTCAAACACATGCGCCACGAGAACGTCATTGGGCTACTGGATGTGTTCA
  CACCTGATGAGTCTCTGGACGACTTCACAGACTTCTACCTGGTGATGCCATTCATGGGCACTGATCTGGG
  CAAACTCATGAAGCATGAGACCCTGAGTGAAGACAGAATCCAGTTTCTTGTGTATCAGATGTTGAAGGGG
  CTGAAGTATATCCATGCGGCTGGTGTCATCCACAGAGACTTGAAGCCTGGCAACCTGGCTGTGAATGAGG
  ACTGTGAGCTGAAGATCCTAGACTTTGGCCTTGCCAGGCAGGCAGACAGTGAGATGACAGGATATGTGGT
  AACCCGGTGGTATCGGGCACCAGAGGTCATCTTGAATTGGATGCGCTACACGCAGACAGTGGACATTTGG
  TCCGTTGGCTGCATCATGGCGGAGATGATTACTGGGAAGATCCTGTTCAAAGGCAATGACCACCTGGACC
  AGCTGAAGGAGATCATGAAGATCACAGGGACGCCCCCTCCTGAGTTTGTTCAGAAGCTACAGAGTGCAGA
  GGCCAAGAACTACATGGAAGGCCTCCCTGAGTTAGAAAAGAAGGATTTTGCCTCTGTCCTGACCAACGCA
  AGCCCTCAGGCTGTGAATCTCCTGGAAAGGATGCTGGTGCTGGATGCGGAACAGCGGGTGACAGCAGCTG
  AGGCGTTAACCCATCCATACTTTGAGTCCCTTCGGGACACTGAGGATGAACCCAAGGCCCAGAAATATGA
  CGACTCCTTTGATGATGTAGACCGCACCCTTGAGGAATGGAAGCGTGTGACTTACAAGGAAGTTCTCAGC
  TTCAAGCCTCCTAGGCAGCTAGGAGCCAGAGTTCCAAAGGAGACGGCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205654 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSPPPARKGFYRQEVTKTAWEVRAVYQDLQPVGSGAYGAVCSAVDSRTGNKVAIKKLYRPFQSELFAKR
  AYRELRLLKHMRHENVIGLLDVFTPDESLDDFTDFYLVMPFMGTDLGKLMKHETLSEDRIQFLVYQMLKG
  LKYIHAAGVIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIW
  SVGCIMAEMITGKILFKGNDHLDQLKEIMKITGTPPPEFVQKLQSAEAKNYMEGLPELEKKDFASVLTNA
  SPQAVNLLERMLVLDAEQRVTAAEALTHPYFESLRDTEDEPKAQKYDDSFDDVDRTLEEWKRVTYKEVLS
  FKPPRQLGARVPKETAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013871
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013871.1, NM_013871.2, NM_013871.3, NP_038899.1
• RefSeq Size: 1905 bp
• RefSeq ORF: 1104 bp
• Locus ID: 29857
• MW: 42 kDa