Acta1 (NM_009606) Mouse Tagged ORF Clone

CAT#: MR205859

Acta1 (Myc-DDK-tagged) - Mouse actin, alpha 1, skeletal muscle (Acta1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acta1
• Synonyms: AA959943; Acta-2; Acts; Actsk-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205859 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGACGAAGACGAGACCACCGCTCTTGTGTGTGACAACGGCTCTGGCCTGGTGAAAGCTGGCTTTG
  CCGGGGATGATGCCCCCAGGGCTGTGTTCCCATCCATCGTGGGCCGACCCCGTCACCAGGGTGTCATGGT
  AGGTATGGGTCAGAAGGACTCCTACGTGGGTGATGAGGCCCAGAGCAAGCGAGGTATCCTGACCCTGAAG
  TACCCCATTGAACATGGCATCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACA
  ATGAGCTGCGTGTGGCCCCTGAGGAGCACCCGACTCTGCTCACCGAGGCCCCCCTGAACCCCAAAGCTAA
  CCGGGAGAAGATGACTCAAATCATGTTTGAGACCTTCAACGTGCCTGCCATGTATGTGGCTATCCAGGCG
  GTGCTGTCCCTCTATGCCTCCGGCCGTACCACCGGCATCGTGTTGGATTCTGGGGACGGTGTCACCCACA
  ACGTGCCCATCTATGAGGGCTATGCCCTGCCACACGCCATCATGCGTCTGGACCTGGCCGGTCGCGACCT
  CACTGACTACCTGATGAAAATCCTCACTGAGCGTGGCTATTCCTTCGTGACCACAGCTGAACGTGAGATT
  GTGCGCGACATCAAAGAGAAGCTGTGCTATGTGGCCCTGGACTTCGAGAATGAGATGGCCACCGCTGCCT
  CTTCCTCCTCCCTGGAGAAGAGCTATGAGCTGCCTGACGGGCAGGTCATCACCATCGGCAATGAGCGTTT
  CCGTTGCCCGGAGACGCTCTTCCAGCCTTCCTTTATCGGTATGGAGTCTGCGGGGATCCATGAGACCACC
  TACAACAGCATCATGAAGTGCGACATCGACATCAGGAAGGACCTGTACGCCAACAACGTCATGTCAGGGG
  GCACCACCATGTACCCTGGTATCGCTGACCGCATGCAGAAGGAGATCACAGCTCTGGCTCCCAGCACCAT
  GAAGATCAAGATCATCGCCCCCCCTGAGCGCAAGTACTCAGTGTGGATCGGTGGCTCCATCCTGGCCTCG
  CTGTCCACCTTCCAGCAGATGTGGATCACCAAGCAGGAGTACGACGAGGCTGGCCCCTCCATTGTGCACC
  GCAAATGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205859 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
  YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
  VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
  VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
  YNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
  LSTFQQMWITKQEYDEAGPSIVHRKCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009606
• ORF Size: 1134 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009606.1, NM_009606.2, NM_009606.3, NP_033736.1
• RefSeq Size: 1481 bp
• RefSeq ORF: 1134 bp
• Locus ID: 11459
• MW: 42.1 kDa
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProtKB/Swiss-Prot Function]