Actc1 (BC062138) Mouse Tagged ORF Clone

CAT#: MR205866

Actc1 (Myc-DDK-tagged) - Mouse actin, alpha, cardiac (cDNA clone MGC:69991 IMAGE:6516862)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actc1
• Synonyms: Actc-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205866 representing BC062138
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGACGATGAGGAGACCACCGCTTTGGTGTGTGACAATGGCTCCGGGCTGGTGAAGGCCGGCTTTG
  CGGGTGATGACGCCCCCAGAGCTGTCTTCCCATCCATCGTGGGTCGCCCACGCCACCAGGGTGTCATGGT
  GGGTATGGGACAGAAGGACTCCTATGTAGGTGATGAAGCCCAGAGCAAGCGAGGTATCCTGACTCTGAAG
  TACCCCATTGAACATGGCATTATCACCAACTGGGATGACATGGAGAAGATCTGGCACCATACATTCTACA
  ATGAGCTCCGTGTGGCCCCAGAGGAACACCCAACCCTGCTCACTGAAGCCCCGCTGAACCCCAAGGCCAA
  CCGTGAGAAGATGACCCAGATCATGTTTGAAACCTTCAATGTGCCTGCCATGTATGTCGCCATCCAGGCA
  GTGCTGTCTCTGTATGCTTCTGGAAGAACCACAGGCATTGTTCTGGATTCTGGCGATGGTGTAACTCATA
  ATGTTCCCATCTATGAGGGTTATGCCTTACCCCATGCCATCATGCGCCTGGATCTAGCTGGTCGGGACCT
  CACTGACTACCTCATGAAGATCCTCACTGAGCGCGGGTACTCCTTTGTCACCACTGCTGAACGTGAAATT
  GTCCGTGACATAAAAGAAAAACTGTGTTACGTCGCCCTGGATTTTGAGAACGAGATGGCCACAGCTGCAT
  CTTCCTCCTCTCTGGAGAAGAGCTATGAACTTCCTGACGGCCAGGTCATCACCATTGGCAACGAGCGCTT
  CCGCTGTCCAGAGACTCTCTTCCAGCCCTCTTTCATTGGTATGGAATCTGCTGGCATTCATGAAACTACT
  TACAACAGCATCATGAAGTGTGACATCGATATCCGCAAAGACCTGTATGCCAACAATGTCCTATCTGGAG
  GCACCACCATGTACCCTGGTATTGCCGATCGTATGCAAAAGGAAATCACTGCACTGGCTCCCAGCACCAT
  GAAGATCAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT
  CTGTCCACCTTCCAGCAAATGTGGATCAGCAAGCAAGAGTATGATGAGGCAGGCCCATCCATTGTCCACC
  GCAAGTGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205866 representing BC062138
  Red=Cloning site Green=Tags(s)
  
  MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
  YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
  VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
  VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
  YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
  LSTFQQMWISKQEYDEAGPSIVHRKCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062138
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC062138.1
• RefSeq Size: 1454
• RefSeq ORF: 1133
• Locus ID: 11464
• MW: 53.2 kDa
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProtKB/Swiss-Prot Function]