Galt (NM_016658) Mouse Tagged ORF Clone

CAT#: MR205914

Galt (Myc-DDK-tagged) - Mouse galactose-1-phosphate uridyl transferase (Galt)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galt
• Synonyms: AW553376
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205914 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGACCTTCCGGGCGAGCGAACACCAGCATATTCGCTACAACCCGCTCCAGGACGAGTGGGTGT
  TAGTGTCGGCTCATCGCATGAAGCGGCCCTGGCAAGGACAAGTGGAGCCCCAGCTTCTAAAGACAGTGCC
  CCGCCACGACCCACTCAACCCTCTGTGTCCCGGGGCCACACGAGCTAATGGGGAGGTGAATCCCCACTAT
  GATGGCACCTTTCTGTTTGACAATGACTTCCCGGCTCTGCAGCCCGATGCTCCGGATCCAGGAACCAGTG
  ACCACCCTCTCTTCCGAGCAGAGGCCGCCAGAGGAGTTTGTAAGGTCATGTGCTTCCACCCCTGGTCGGA
  TGTGACGCTGCCACTCATGTCTGTCCCTGAGATCCGAGCTGTCATCGATGCATGGGCCTCAGTAACAGAG
  GAGCTGGGTGCCCAGTACCCTTGGGTGCAGATCTTTGAAAATAAAGGAGCCATGATGGGCTGTTCTAACC
  CCCATCCCCACTGCCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCCAGCGTGAAGAGCGATCCCA
  GCAGACCTATCACAGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCACCAAGAGCTCCTCAGGAAG
  GAACGTCTGGTCCTAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGGGCAGTGTGGCCTTTCCAGA
  CACTTCTGCTGCCCCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCGCTGAGCGTGATGATCTCGC
  CTCCATCATGAAGAAGCTCTTGACCAAGTACGACAATCTATTTGAGACATCCTTTCCCTACTCCATGGGC
  TGGCATGGGGCTCCCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGGCAGCTCCACGCCCACTACT
  ACCCCCCACTTCTGCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATGAAATGCTTGCCCAGGCCCA
  GCGTGACCTCACTCCCGAACAGGCCGCAGAAAGATTAAGGGCACTTCCCGAGGTACACTATTGCCTGGCG
  CAGAAAGACAAGGAAACGGCAGCCATTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205914 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATFRASEHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGATRANGEVNPHY
  DGTFLFDNDFPALQPDAPDPGTSDHPLFRAEAARGVCKVMCFHPWSDVTLPLMSVPEIRAVIDAWASVTE
  ELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQTYHSQHGKPLLLEYGHQELLRK
  ERLVLTSEHWIVLVPFWAVWPFQTLLLPRRHVRRLPELNPAERDDLASIMKKLLTKYDNLFETSFPYSMG
  WHGAPTGLKTGATCDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYCLA
  QKDKETAAIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016658
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2000 bp
• RefSeq ORF: 1083 bp
• Locus ID: 14430
• MW: 41.2 kDa
• Gene Summary: The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]