Galt (NM_016658) Mouse Tagged ORF Clone
CAT#: MR205914
- TrueORF®
Galt (Myc-DDK-tagged) - Mouse galactose-1-phosphate uridyl transferase (Galt)
"NM_016658" in other vectors (2)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Galt |
Synonyms | AW553376 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR205914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGACCTTCCGGGCGAGCGAACACCAGCATATTCGCTACAACCCGCTCCAGGACGAGTGGGTGT TAGTGTCGGCTCATCGCATGAAGCGGCCCTGGCAAGGACAAGTGGAGCCCCAGCTTCTAAAGACAGTGCC CCGCCACGACCCACTCAACCCTCTGTGTCCCGGGGCCACACGAGCTAATGGGGAGGTGAATCCCCACTAT GATGGCACCTTTCTGTTTGACAATGACTTCCCGGCTCTGCAGCCCGATGCTCCGGATCCAGGAACCAGTG ACCACCCTCTCTTCCGAGCAGAGGCCGCCAGAGGAGTTTGTAAGGTCATGTGCTTCCACCCCTGGTCGGA TGTGACGCTGCCACTCATGTCTGTCCCTGAGATCCGAGCTGTCATCGATGCATGGGCCTCAGTAACAGAG GAGCTGGGTGCCCAGTACCCTTGGGTGCAGATCTTTGAAAATAAAGGAGCCATGATGGGCTGTTCTAACC CCCATCCCCACTGCCAGGTTTGGGCTAGCAGCTTCCTGCCAGATATCGCCCAGCGTGAAGAGCGATCCCA GCAGACCTATCACAGCCAGCATGGAAAACCTTTGTTATTGGAATATGGTCACCAAGAGCTCCTCAGGAAG GAACGTCTGGTCCTAACCAGTGAGCACTGGATAGTTCTGGTCCCCTTCTGGGCAGTGTGGCCTTTCCAGA CACTTCTGCTGCCCCGGCGGCACGTGCGGCGGCTACCTGAGCTGAACCCCGCTGAGCGTGATGATCTCGC CTCCATCATGAAGAAGCTCTTGACCAAGTACGACAATCTATTTGAGACATCCTTTCCCTACTCCATGGGC TGGCATGGGGCTCCCACGGGATTAAAGACTGGAGCCACCTGTGACCACTGGCAGCTCCACGCCCACTACT ACCCCCCACTTCTGCGATCCGCAACTGTCCGGAAGTTCATGGTTGGCTATGAAATGCTTGCCCAGGCCCA GCGTGACCTCACTCCCGAACAGGCCGCAGAAAGATTAAGGGCACTTCCCGAGGTACACTATTGCCTGGCG CAGAAAGACAAGGAAACGGCAGCCATTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205914 protein sequence
Red=Cloning site Green=Tags(s) MAATFRASEHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGATRANGEVNPHY DGTFLFDNDFPALQPDAPDPGTSDHPLFRAEAARGVCKVMCFHPWSDVTLPLMSVPEIRAVIDAWASVTE ELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQTYHSQHGKPLLLEYGHQELLRK ERLVLTSEHWIVLVPFWAVWPFQTLLLPRRHVRRLPELNPAERDDLASIMKKLLTKYDNLFETSFPYSMG WHGAPTGLKTGATCDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYCLA QKDKETAAIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016658 |
ORF Size | 1083 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 2000 bp |
RefSeq ORF | 1083 bp |
Locus ID | 14430 |
MW | 41.2 kDa |
Gene Summary | The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR205914L3 | Lenti ORF clone of Galt (Myc-DDK-tagged) - Mouse galactose-1-phosphate uridyl transferase (Galt) |
USD 550.00 |
|
MR205914L4 | Lenti ORF clone of Galt (mGFP-tagged) - Mouse galactose-1-phosphate uridyl transferase (Galt) |
USD 550.00 |
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