Mapkapk2 (NM_008551) Mouse Tagged ORF Clone

CAT#: MR206027

Mapkapk2 (Myc-DDK-tagged) - Mouse MAP kinase-activated protein kinase 2 (Mapkapk2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mapkapk2
• Synonyms: AA960234; MAPKAP-K2; MK-2; MK2; Rps6kc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206027 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCGGGCTCTCCGGGCCAGACTCCGCCGGCGCCTTTCCCCAGCCCTCCACCGCCAGCCCCGGCCC
  AGCCGCCGCCGCCGTTCCCCCAGTTCCACGTCAAGTCGGGCCTGCAGATCCGAAAGAACGCCATCACCGA
  CGACTACAAGGTCACCAGCCAAGTGCTTGGCCTGGGCATCAACGGGAAGGTGCTGCGGATCTTCGACAAG
  AGAACCCAGCAAAAATTCGCCCTAAAGATGCTCCAGGACTGTCCGAAGGCGCGCAGAGAGGTGGAGCTGC
  ACTGGAGGGCCTCCCAGTGCCCACACATTGTGCACATCGTGGATGTCTATGAGAACCTGTATGCCGGGAG
  GAAGTGCCTGCTGATTGTCATGGAGTGTCTCGATGGTGGAGAGCTCTTTAGTCGAATCCAGGACCGAGGA
  GACCAGGCATTCACAGAAAGAGAGGCGTCAGAGATCATGAAGAGCATCGGCGAGGCCATCCAGTACCTGC
  ACTCGATCAACATTGCTCACCGGGATGTCAAGCCTGAGAACCTCTTATATACTTCCAAAAGGCCCAATGC
  CATTTTGAAACTCACTGATTTTGGCTTTGCCAAGGAAACCACCAGTCACAACTCTTTGACCACTCCGTGT
  TATACACCATACTATGTGGCTCCGGAAGTCCTGGGCCCGGAGAAGTATGACAAGTCCTGTGACATGTGGT
  CCTTGGGTGTCATCATGTATATTTTGCTGTGTGGGTATCCCCCCTTCTATTCCAATCACGGCCTTGCCAT
  CTCTCCGGGCATGAAGACTCGTATTCGAATGGGCCAGTATGAATTTCCTAACCCGGAGTGGTCAGAAGTA
  TCAGAAGAAGTGAAGATGCTTATCCGGAATCTGCTAAAAACAGAGCCCACCCAGAGAATGACCATCACAG
  AATTCATGAACCACCCCTGGATCATGCAATCTACGAAGGTCCCTCAGACTCCACTGCACACCAGCCGTGT
  CCTGAAGGAGGACAAGGAACGATGGGAGGATGTCAAGGAGGAGATGACCAGTGCCTTGGCCACGATGCGT
  GTTGACTATGAGCAGATCAAGATAAAGAAGATAGAAGACGCATCCAACCCTCTGCTTCTCAAGAGGCGGA
  AGAAAGCTCGTGCTGTGGAGGATGCGGCTCTCGCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206027 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSGSPGQTPPAPFPSPPPPAPAQPPPPFPQFHVKSGLQIRKNAITDDYKVTSQVLGLGINGKVLRIFDK
  RTQQKFALKMLQDCPKARREVELHWRASQCPHIVHIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRG
  DQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPC
  YTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEV
  SEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSALATMR
  VDYEQIKIKKIEDASNPLLLKRRKKARAVEDAALAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008551
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008551.1, NM_008551.2, NP_032577.1
• RefSeq Size: 2586 bp
• RefSeq ORF: 1161 bp
• Locus ID: 17164
• MW: 44 kDa