Bcat1 (NM_007532) Mouse Tagged ORF Clone
CAT#: MR206032
- TrueORF®
Bcat1 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2
"NM_007532" in other vectors (3)
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Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Bcat1 |
Synonyms | BCATc; Eca39 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR206032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACTGCAGTAATGGATGCTCCGCGCCGTTTGCTGGAGAAAGAGGATCAGAAGAAGTGGCGGAGA CTTTTAGGGCCAAAGATCTCATCATCACACCAGCCACTGTCTTAAAAGAGAAGCCGGACCCGGATTCGCT GGTCTTTGGAGCTACGTTTACTGACCACATGCTGACGGTGGAGTGGTCCTCTGCGTCTGGATGGGAGAAA CCTCACATTAAGCCTTTTGGAAACTTGCCCATACATCCCGCTGCCTCTGTTTTGCACTACGCTGTGGAAC TGTTTGAAGGCTTGAAAGCCTTTCGGGGAGTTGATAACAAGATCCGATTGTTCCGGCCGGACCTCAACAT GGATAGAATGTGCCGATCTGCTGTGAGGACCACGCTGCCGATGTTTGACAAGGAGGAGCTCCTAAAGTGT ATTCTTCAGCTTCTACAGATCGACCAAGAATGGGTTCCCTACTCCACCTCTGCCAGCCTCTACATCCGCC CCACATTTATCGGAACTGAGCCATCTCTTGGCGTCAAGAAGCCTTCCAAAGCCCTACTCTTTGTGATCCT GAGCCCCGTGGGACCTTATTTTTCTAGTGGATCTTTTACTCCGGTGTCCCTGTGGGCCAATCCAAAGTAT ATCAGAGCCTGGAAAGGTGGGACTGGAGACTGCAAGATGGGCGGCAATTATGGAGCCTCCCTTCTGGCAC AGTGCGAGGCCGCGGAGAATGGCTGTCAGCAGGTCCTGTGGCTGTACGGCAAGGACAACCAGATAACTGA AGTAGGCACAATGAATCTTTTCCCCTACTGGATAAACGAAGACGGAGAAGAGGAGCTGGCAACGCCCCCA CTAGATGGCATCATTCTCCCAGGAGTGACCAGGCAGAGCATCCTGGAGCTGGCACAACAGTGGGGTGAGT TTAAGGTATGTGAGAGACACCTCACCATGGATGACCTGGCCACCGCCCTTGAGGGGAACAGAGTGAAGGA GATGTTCGGCTCAGGGACAGCCTGCGTTGTCTGCCCAGTCTCTGATATTCTGTACAAGGGCCAGATGCTG CATATTCCAACGATGGAGAATGGCCCCAAGCTTGCAAGTCGAATCCTGGGAAAGCTGACTGATATCCAGT ATGGAAGGGTGGAGAGTGACTGGACAATCGAGCTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206032 protein sequence
Red=Cloning site Green=Tags(s) MKDCSNGCSAPFAGERGSEEVAETFRAKDLIITPATVLKEKPDPDSLVFGATFTDHMLTVEWSSASGWEK PHIKPFGNLPIHPAASVLHYAVELFEGLKAFRGVDNKIRLFRPDLNMDRMCRSAVRTTLPMFDKEELLKC ILQLLQIDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPSKALLFVILSPVGPYFSSGSFTPVSLWANPKY IRAWKGGTGDCKMGGNYGASLLAQCEAAENGCQQVLWLYGKDNQITEVGTMNLFPYWINEDGEEELATPP LDGIILPGVTRQSILELAQQWGEFKVCERHLTMDDLATALEGNRVKEMFGSGTACVVCPVSDILYKGQML HIPTMENGPKLASRILGKLTDIQYGRVESDWTIELP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007532 |
ORF Size | 1161 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007532.1, NM_007532.2, NM_007532.3, NM_007532.4, NM_007532.5, NP_031558.3 |
RefSeq Size | 7868 bp |
RefSeq ORF | 1161 bp |
Locus ID | 12035 |
MW | 42.7 kDa |
Gene Summary | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202068 | Bcat1 (untagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2, (10ug) |
USD 210.00 |
|
MR206032L3 | Lenti ORF clone of Bcat1 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2 |
USD 500.00 |
|
MR206032L4 | Lenti ORF clone of Bcat1 (mGFP-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2 |
USD 500.00 |
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