Pipox (NM_008952) Mouse Tagged ORF Clone

CAT#: MR206088

Pipox (Myc-DDK-tagged) - Mouse pipecolic acid oxidase (Pipox)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pipox
• Synonyms: LPIPOX; Pso
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206088 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCAGACAGATTTCTGGGATGCTATTGTGATTGGAGCAGGCATCCAGGGCTGCTTTACCGCGT
  ACCACCTGGCCAAACACTCCAAGAGCGTCCTCCTGCTGGAGCAGTTCTTTCTTCCCCATTCCCGAGGAAG
  CTCACATGGACAGAGCCGCATAATCAGAAAGGCTTATCCAGAGGACTTCTACACGATGATGATGAAGGAA
  TGTTATCAGACATGGGCCCAGCTGGAGCGTGAAGCCGGAACCCAGTTACACAGGCAGACTGAGCTCTTAC
  TTCTGGGGACGAAGGAAAATCCAGGATTAAAGACAATCCAGGCCACCCTGTCTAGGCAAGGGATCGACCA
  TGAGTATCTTTCGTCAGTGGATTTAAAGCAACGCTTTCCAAACATTCGGTTCACCAGGGGAGAAGTGGGG
  CTCTTGGACAAGACTGGAGGTGTCCTCTATGCAGACAAGGCCCTCAGAGCCCTCCAGCATATAATTTGTC
  AGCTAGGAGGCACTGTGTGTGATGGAGAGAAGGTAGTGGAGATAAGACCTGGTCTACCCGTCACAGTGAA
  AACCACCTTGAAGAGCTACCAAGCCAACAGCTTGGTCATCACTGCTGGTCCCTGGACCAACCGGCTTCTG
  CATCCTCTGGGGATTGAGTTGCCTCTCCAGACCCTTAGGATCAATGTGTGTTACTGGCGAGAGAAGGTCC
  CTGGGAGCTATGGTGTATCACAAGCTTTTCCATGCATCCTGGGTCTGGATCTGGCCCCCCACCACATCTA
  TGGACTGCCAGCATCCGAGTACCCGGGGCTGATGAAGATCTGCTATCACCATGGTGACAATGTGGACCCA
  GAGGAGCGGGACTGCCCCAAAACCTTCTCAGACATCCAAGACGTTCAAATTCTATGCCACTTTGTCAGAG
  ATCATTTACCAGGCCTGCGGGCTGAGCCAGACATCATGGAACGCTGCATGTACACGAATACTCCTGATGA
  GCACTTTATTCTTGATTGCCACCCAAAGTATGACAACATTGTCATCGGCGCTGGATTCTCTGGACATGGA
  TTCAAGTTGGCCCCTGTTGTGGGGAAGATCCTCTATGAGCTAAGCATGAAATTACCCCCATCCTATGACT
  TGGCTCCGTTTCGAATGAGTCGCTTCTCTACGCTGAGCAAAGCCCACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206088 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAQTDFWDAIVIGAGIQGCFTAYHLAKHSKSVLLLEQFFLPHSRGSSHGQSRIIRKAYPEDFYTMMMKE
  CYQTWAQLEREAGTQLHRQTELLLLGTKENPGLKTIQATLSRQGIDHEYLSSVDLKQRFPNIRFTRGEVG
  LLDKTGGVLYADKALRALQHIICQLGGTVCDGEKVVEIRPGLPVTVKTTLKSYQANSLVITAGPWTNRLL
  HPLGIELPLQTLRINVCYWREKVPGSYGVSQAFPCILGLDLAPHHIYGLPASEYPGLMKICYHHGDNVDP
  EERDCPKTFSDIQDVQILCHFVRDHLPGLRAEPDIMERCMYTNTPDEHFILDCHPKYDNIVIGAGFSGHG
  FKLAPVVGKILYELSMKLPPSYDLAPFRMSRFSTLSKAHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008952
• ORF Size: 1173 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008952.1, NM_008952.2, NP_032978.1
• RefSeq Size: 1774 bp
• RefSeq ORF: 1173 bp
• Locus ID: 19193
• MW: 43.8 kDa