Tsg101 (NM_021884) Mouse Tagged ORF Clone

CAT#: MR206104

Tsg101 (Myc-DDK-tagged) - Mouse tumor susceptibility gene 101 (Tsg101)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tsg101
• Synonyms: AI255943; CC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206104 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGTCCGAGAGTCAGCTGAAGAAGATGATGTCCAAGTACAAATATAGAGATCTAACCGTCCGTC
  AAACTGTCAATGTCATCGCTATGTACAAAGATCTCAAACCTGTATTGGATTCATATGTTTTTAATGATGG
  CAGTTCCAGGGAGCTGGTGAACCTCACTGGTACAATCCCAGTGCGTTATCGAGGTAATATATATAATATT
  CCAATATGCCTGTGGCTGCTGGACACATACCCATATAACCCCCCTATCTGTTTTGTTAAGCCTACTAGTT
  CAATGACTATTAAAACAGGAAAGCATGTGGATGCAAATGGGAAAATCTACCTACCTTATCTACATGACTG
  GAAACATCCACGGTCAGAGTTGCTGGAGCTTATTCAAATCATGATTGTGATATTTGGAGAGGAGCCTCCA
  GTGTTCTCCCGGCCTACTGTTTCTGCATCCTACCCACCATACACAGCAACAGGGCCACCAAATACCTCCT
  ACATGCCAGGCATGCCAAGTGGAATCTCTGCATATCCATCTGGATACCCTCCCAACCCCAGTGGTTATCC
  TGGCTGTCCTTACCCACCTGCTGGCCCATACCCTGCCACAACAAGCTCACAGTACCCTTCCCAGCCTCCT
  GTGACCACTGTTGGTCCCAGCAGAGATGGCACAATCAGTGAGGACACTATCCGTGCATCTCTCATCTCAG
  CAGTCAGTGACAAACTGAGATGGCGGATGAAGGAGGAAATGGATGGTGCCCAGGCAGAGCTTAATGCCTT
  GAAACGAACAGAGGAAGATCTGAAAAAAGGCCACCAGAAACTGGAAGAGATGGTCACCCGCTTAGATCAA
  GAAGTAGCTGAAGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAGAACTAAGTTCTGCTCTGG
  AGAAAATGGAAAATCAATCTGAAAATAATGATATTGATGAAGTTATCATTCCCACAGCCCCACTGTATAA
  ACAGATTCTAAATCTGTATGCAGAGGAAAATGCTATTGAAGACACTATCTTTTACCTTGGAGAAGCTTTG
  CGGCGGGGAGTCATAGACCTGGATGTGTTCCTGAAACACGTCCGCCTCCTGTCCCGTAAACAGTTCCAGC
  TAAGGGCACTAATGCAAAAGGCAAGGAAGACTGCGGGCCTTAGTGACCTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206104 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVSESQLKKMMSKYKYRDLTVRQTVNVIAMYKDLKPVLDSYVFNDGSSRELVNLTGTIPVRYRGNIYNI
  PICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHDWKHPRSELLELIQIMIVIFGEEPP
  VFSRPTVSASYPPYTATGPPNTSYMPGMPSGISAYPSGYPPNPSGYPGCPYPPAGPYPATTSSQYPSQPP
  VTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDGAQAELNALKRTEEDLKKGHQKLEEMVTRLDQ
  EVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEAL
  RRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021884
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021884.1, NM_021884.2, NM_021884.3, NM_021884.4, NP_068684.1
• RefSeq Size: 1832 bp
• RefSeq ORF: 1176 bp
• Locus ID: 22088
• MW: 44.1 kDa