Terf2ip (NM_020584) Mouse Tagged ORF Clone

CAT#: MR206137

Terf2ip (Myc-DDK-tagged) - Mouse telomeric repeat binding factor 2, interacting protein (Terf2ip)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Terf2ip
• Synonyms: Rap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206137 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGATGGATCTGGGTAAAGACCCCAATGGGCCCACTCACTCCTCCACTCTGTTCGTGAGAG
  AAGACGGCAGCGCCATGTCGTTTTACGTGCGGCCCAGCTCGGCCAAGCGCCGGCTGTCGACGCTCATCCT
  GCACGGCGGCGGCACCGTGTGTCGGGTGCAGGAACCCGGAGCCGTGCTTCTCGCCCAGCCCGGGGAGGCG
  CTGGCCGAGGCTTCGGGCGACTTCATCTCCACGCAGTACATCCTAGACTGCGTGGATCGCAACGAGAAGC
  TGGACCTGGAGGCCTATCGGCTGGGCCTGACGGAGCAGGCGTCCGATCCGAAGCCCGGGGCTTCCACCGA
  GGGCTCCACGGAACCGGAGCCGCAGCCCCTGACCGGGCGCATCGCCTACACCGACGCGGAAGATGTGGCC
  ATCCTGACCTACGTGAAGGAGAACGCCCGTTCGCCCAGCTCGGTCACAGGCAATGCCTTGTGGAAAGCGA
  TGGAGAAGAGCTCGCTCACGCAGCACTCCTGGCAGTCGCTCAAGGACCGCTACCTCAAGCACCTACGGGG
  TCAGGAGCACAAGTACCTGCTCGGGAACGCCCCGGTCAGCCCGTCCTCCCAGAAGCTCAAACGGAAGGCG
  GAGCAGGACCCCGAGGCCGCGGATAGCGGAGAGCCACAGAACAAGAGAGCGCCAGACTTGCCTGAGGAGG
  AGTGTGTGAAAGGAGAGATCAAGGAGAATGGAGAGGCAGACAACAAGCTGTTTGAGGAAGCCGCTCCGGA
  GTTCGGAGAAGCCGTGGTGGATGAGAGCCCTGACTTTGAAATACATATAACGATGTGTGATGGTGATCCA
  CCCACACCCGAGGAAGACTCAGAAACACAGCCAGACGAGGAGGAAGAAGAACCAAAAGTTTCTACGCAAG
  AAGTGGGAACTGCCATTAAGGTGATCCGGCAGCTAATGGAGAAGTTCAACTTGGATCTATCAACAGTTAC
  ACAGGCCTTGCTGAAGAACAGTGGTGAGCTGGAGGCCACGTCCTCCTTCTTAGAGTCGGGGCGGAGACCC
  GACGGTTATCCCATTTGGTGCAGACAAGATGACTTAGATTTGCAGAAGGACGATGACGACACGAAAAATG
  CACTGGTCAAAAAGTTTGGAGCTCAGAACGTTGCTCGGAGGATTGAATTCCGAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206137 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAMDLGKDPNGPTHSSTLFVREDGSAMSFYVRPSSAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEA
  LAEASGDFISTQYILDCVDRNEKLDLEAYRLGLTEQASDPKPGASTEGSTEPEPQPLTGRIAYTDAEDVA
  ILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGNAPVSPSSQKLKRKA
  EQDPEAADSGEPQNKRAPDLPEEECVKGEIKENGEADNKLFEEAAPEFGEAVVDESPDFEIHITMCDGDP
  PTPEEDSETQPDEEEEEPKVSTQEVGTAIKVIRQLMEKFNLDLSTVTQALLKNSGELEATSSFLESGRRP
  DGYPIWCRQDDLDLQKDDDDTKNALVKKFGAQNVARRIEFRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020584
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020584.2, NP_065609.2
• RefSeq Size: 3599 bp
• RefSeq ORF: 1182 bp
• Locus ID: 57321
• MW: 43.4 kDa