Asah1 (NM_019734) Mouse Tagged ORF Clone

CAT#: MR206162

Asah1 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 1 (Asah1)

Product Images

Coomassie blue staining of purified Asah1 protein (Cat# TP506162). The protein was produced from HEK293T cells transfected with Asah1 cDNA clone (Cat# MR206162) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asah1
• Synonyms: 2310081N20Rik; AC; Asah
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206162 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCCAAAGTCTTCTCACCTGGGTCCTAGCCGCGGCAGTCACCTGCGCCCAGGCACAGGATGTGC
  CGCCGTGGACAGAAGATTGCAGAAAATCAACTTATCCTCCTTCTGGACCAACCTATAGAGGACCAGTTCC
  GTGGCACACCATAAATCTTGATTTACCACCCTACAAAAGATGGCATGAATTATTGGCTCAAAAGGCACCA
  GCGTTGAGGATTTTAGTGAATTCCATAACGAGTTTAGTGAATACATTTGTGCCAAGTGGAAAACTAATGA
  AGATGGTGGATCAAAAGCTGCCTGGTATGATTGGCAGCCTTCCTGACCCTTTTGGAGAGGAAATGAGGGG
  AATTGCAGATGTTACTGGGATTCCTCTAGGAGAGATTATTTCATTCAACATTTTCTATGAATTGTTTACC
  ATGTGTACATCAATCATAACTGAAGATGAGAAAGGTCATTTACTACATGGGAGAAACATGGATTTTGGAA
  TATTTCTTGGGTGGAATATAAATAATAACACTTGGGTTGTCACAGAAGAATTAAAGCCCTTAACAGTGAA
  TTTGGACTTCCAAAGAAACAATAAGACTGTTTTCAAGGCTACAAGTTTTGTTGGATATGTGGGCATGTTG
  ACAGGATTCAAACCAGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGG
  GTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGA
  AAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTT
  ATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCT
  ATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTT
  GTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGAATCTCTCCTTT
  GCTACCATCTATGATGTCCTATCAACAAAACCTGTCCTCAACAAGCTGACTGTATTCACAACCTTGATGG
  ATGTTACCAAAGGTCAATTTGAAAGTCACCTTCGAGATTGCCCAGACCCTTGTATAGGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206162 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGQSLLTWVLAAAVTCAQAQDVPPWTEDCRKSTYPPSGPTYRGPVPWHTINLDLPPYKRWHELLAQKAP
  ALRILVNSITSLVNTFVPSGKLMKMVDQKLPGMIGSLPDPFGEEMRGIADVTGIPLGEIISFNIFYELFT
  MCTSIITEDEKGHLLHGRNMDFGIFLGWNINNNTWVVTEELKPLTVNLDFQRNNKTVFKATSFVGYVGML
  TGFKPGLFSLSLNERFSINGGYLGILEWMFGRKDAQWVGFITRSVLENTTSYEEAKNTLTKTKIMAPVYF
  ILGGKKSGEGCVITRERKESLDVYELDPKHGRWYVVQTNYDRWKNTLFIDDRRTPAKKCLNHTTQKNLSF
  ATIYDVLSTKPVLNKLTVFTTLMDVTKGQFESHLRDCPDPCIGW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019734
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019734.1, NM_019734.2, NM_019734.3, NP_062708.1
• RefSeq Size: 2251 bp
• RefSeq ORF: 1185 bp
• Locus ID: 11886
• MW: 44.7 kDa
• Gene Summary: This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype. [provided by RefSeq, Oct 2015]