Ipmk (NM_027184) Mouse Tagged ORF Clone

CAT#: MR206205

Ipmk (Myc-DDK-tagged) - Mouse inositol polyphosphate multikinase (Ipmk)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ipmk
• Synonyms: 2410017C19Rik; AA408208; Impk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206205 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGAGCCCCCAGCGCTGCGCCTGCGGCCGCCGGGAAGCACCGGAGACAGCCCGCCGGTGCCGC
  GCCTGCTCGGAGGCTGCGTGCCGCTGTCGCATCAGGTGGCTGGCCACATGTACGGCAAGGACAAAGTGGG
  CATACTCCAGCACCCAGATGGTACAGTTCTGAAACAGCTACAGCCACCGCCACGGGGCCCAAGAGAGCTG
  GAATTTTATACCATGGTTTACGCTGCTGACTGTGCCGATGCTGTTCTCCTGGAGCTGCGAAAACACCTGC
  CCAAATACTACGGCGTCTGGTCCCCTCCCACCGCACCAAACGATGTGTACCTAAAACTGGAAGATGTGAC
  TCATAAGTTTAACAAACCCTGTATAATGGACGTGAAGATTGGGCGGAAGAGCTACGACCCCTTTGCGTCA
  TCAGAGAAGATTCAGCAGCAGGTCAGCAAGTACCCTCTGATGGAGGAGATCGGGTTCCTGGTGCTCGGCA
  TGAGGGTTTATCATCTTCACTCTGACAGCTACGAGACACAAAACCAGCACTATGGAAGAGGCCTAACGAA
  AGAGACCCTGAAGGAAGGCGTCTCCAAGTTTTTCCACAATGGCTTCTGTTTAAGAAAAGATGCGATTGCC
  GCCAGTATTCAGAAGGTAGAGAAGATTCTCCAGTGGTTTGAAAATCAGAAGCAGCTTAACTTTTACGCAA
  GTTCTTTACTGTTTGTTTATGAAGGTTCATCTCAGCCAGCTACTACAAAAGCAAACGATAGAACTTTGGC
  GGGGAGGTTTCTCTCCAAAGGACCCCTGACGGATGCGGACGGCCTGGAGTGCAATAACAACTTCCACCTG
  TTCGGCGCCCCGCCCAACGGGATGTCGGTGGGCAAGAGCTTATCGAAGGCGTACTCGAGGCACAGAAAGC
  TGTATGCAAAAAAGCACCAGAGTCAGACTTCCCTGAAAGTCGAAACGCTGGAGCAAGACAACGGGTGGAG
  AAGCATGTCCCAGGAACACTTAAACGGAAACGTGCTTGCCCAACTGGAAAAGGTGTTCTACCACCTTCCC
  GCGGGTCGTCCGGAGATCCCGGAAGCGGAAGTACGGATGATAGACTTTGCTCACGTGTTCCCTAGCAACA
  CAGTCGATGAGGGGTATGTTTACGGTCTGAAGCATCTAATTGCCGTGCTTCGGAGTATTTTAGACAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206205 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEPPALRLRPPGSTGDSPPVPRLLGGCVPLSHQVAGHMYGKDKVGILQHPDGTVLKQLQPPPRGPREL
  EFYTMVYAADCADAVLLELRKHLPKYYGVWSPPTAPNDVYLKLEDVTHKFNKPCIMDVKIGRKSYDPFAS
  SEKIQQQVSKYPLMEEIGFLVLGMRVYHLHSDSYETQNQHYGRGLTKETLKEGVSKFFHNGFCLRKDAIA
  ASIQKVEKILQWFENQKQLNFYASSLLFVYEGSSQPATTKANDRTLAGRFLSKGPLTDADGLECNNNFHL
  FGAPPNGMSVGKSLSKAYSRHRKLYAKKHQSQTSLKVETLEQDNGWRSMSQEHLNGNVLAQLEKVFYHLP
  AGRPEIPEAEVRMIDFAHVFPSNTVDEGYVYGLKHLIAVLRSILDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027184
• ORF Size: 1191 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027184.1, NM_027184.2, NP_081460.1
• RefSeq Size: 5432 bp
• RefSeq ORF: 1191 bp
• Locus ID: 69718
• MW: 44.5 kDa