Gtf2h2 (NM_022011) Mouse Tagged ORF Clone

CAT#: MR206208

  • TrueORF®

Gtf2h2 (Myc-DDK-tagged) - Mouse general transcription factor II H, polypeptide 2 (Gtf2h2)


  "NM_022011" in other vectors (4)


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Reconstitution Protocol

USD 350.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Gtf2h2
Synonyms 44kDa; BTF2 p44; BTF2-p44; Btf2p44; p44
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206208 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAAGAACCTGAGAGAACCAAGCGGTGGGAAGGAGGCTATGAGAGAACCTGGGAAATTCTTAAAG
AAGATGAAACTGGATCACTTAAAGCTACAATAGAAGATATTCTCTTCAAGGCAAAGAGGAAAAGAGTGTT
TGAGCACCATGGACAAGTCCGACTTGGAATGATGCGCCACCTGTATGTGGTGGTGGATGGATCGAGAACA
ATGGAAGATCAGGATTTAAAGCCCAATAGACTGACTTGCACTTTAAAGTTGCTGGAATACTTTGTAGAAG
AATATTTTGATCAAAACCCTATCAGTCAGATTGGAATAATTGTAACTAAGAGTAAAAGAGCTGAAAAACT
GACTGAACTCTCAGGAAACCCAAGGAAACATATAACATCTTTGAAGAAAGCTGTAGATATGACCTGCCAT
GGAGAACCATCGCTCTATAATTCCTTAAGCATGGCTATGCAGACCCTAAAACACATGCCTGGACATACAA
GTAGAGAAGTGCTCATCATCTTCAGTAGCCTCACCACCTGTGATCCATCTAATATTTACGATCTCATCAA
GACCCTGAAGACAGCTAAAATTAGAGTGTCTGTTATTGGATTATCTGCGGAGGTTCGAGTTTGTACTGTA
CTTGCTCGTGAAACTGGTGGCACATACCATGTTATCTTAGATGAAACCCATTACAAGGAGTTGTTGGCAC
ATCATGTGAGCCCCCTTCCTGCCAGCTCAAGCTCCGAGTGCTCACTCATTCGCATGGGATTCCCTCAGCA
TACCATTGCTTCTTTGTCTGATCAGGATGCAAAACCATCCTTCAGCATGGCGCATTTGGATAACAACAGC
ACTGAGCCAGGGCTTACACTGGGAGGCTACTTCTGCCCACAGTGCCGAGCAAAGTACTGCGAGCTTCCTG
TTGAATGTAAAATATGTGGTCTTACTTTGGTGTCTGCACCTCATTTGGCAAGATCTTACCATCATTTATT
TCCTTTGGATGCTTTTCAAGAAATTTCCCTAGAAGAATATAAAGGAGAAAGGTTTTGTTATGGATGTCAG
GGGGAATTGAAAGACCAACATGTCTATGTTTGCACAGTGTGCCAAAATGTTTTTTGTGTGGACTGTGATG
TCTTTGTTCATGACTCTCTCCATTGTTGTCCTGGCTGTATTCATAAGATCCCAACTCCTTCAGGTATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206208 protein sequence
Red=Cloning site Green=Tags(s)

MDEEPERTKRWEGGYERTWEILKEDETGSLKATIEDILFKAKRKRVFEHHGQVRLGMMRHLYVVVDGSRT
MEDQDLKPNRLTCTLKLLEYFVEEYFDQNPISQIGIIVTKSKRAEKLTELSGNPRKHITSLKKAVDMTCH
GEPSLYNSLSMAMQTLKHMPGHTSREVLIIFSSLTTCDPSNIYDLIKTLKTAKIRVSVIGLSAEVRVCTV
LARETGGTYHVILDETHYKELLAHHVSPLPASSSSECSLIRMGFPQHTIASLSDQDAKPSFSMAHLDNNS
TEPGLTLGGYFCPQCRAKYCELPVECKICGLTLVSAPHLARSYHHLFPLDAFQEISLEEYKGERFCYGCQ
GELKDQHVYVCTVCQNVFCVDCDVFVHDSLHCCPGCIHKIPTPSGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022011
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022011.1, NM_022011.2, NM_022011.3, NM_022011.4, NP_071294.2
RefSeq Size 1707 bp
RefSeq ORF 1191 bp
Locus ID 23894
MW 44.7 kDa

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