Tm7sf2 (BC014769) Mouse Tagged ORF Clone

CAT#: MR206271

Tm7sf2 (Myc-DDK-tagged) - Mouse transmembrane 7 superfamily member 2 (cDNA clone MGC:25957 IMAGE:4239159)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tm7sf2
• Synonyms: ANG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206271 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTCGTGAGGCCTCCCAGGCCCCACTGGAATTCGGGGGGCCGTTGGGCGTCGCGGCTTTACTGA
  TCCTGCTGCCTGCCACCATGTTCCACCTGCTCCTGGCGGCTCGCTCGGGCCCGGCGCGCCTCCTGGCCCT
  ACCGGCCTATCTGCCTGGGCTGGAGGAGCTGTGGAGCCCATGGGCTCTGCTGCTATTGTTCATCTGGCTC
  GGCCTGCAGGTGGCGCTCTATTTGCTGCCTGCACGCAAGGTGGCCGAAGGCCTGGAACTGAAGGACAAGA
  GTCGCCTGCGCTACCCTATTAATGGCTTCCAGGCTCTGGTGCTAACAGCCCTGTTGATGGGGCTGGGGGT
  GTCAGTGGGGCTGCCCCTGGGGGCACTCCCTGGAATGCTCCTGCCCTTGGCCTTTGCGACCACTCTCACC
  AGCTTTATCTTCAGCCTCCTCCTCTATGCGAAGGCTTTGGTAGCTCCTGCCTCAGCCCTGGCTCCTGGGG
  GAAACTCAGGAAATTCCATGTATGACTTCTTTCTTGGACGGGAGCTGAACCCTCGCCTCGGTTCCTTTGA
  CTTCAAATATTTCTGTGAGCTGAGACCTGGCCTCATTGGCTGGGTTTTCATTAACCTGGCCCTGCTGATG
  CAGGAGGCAGAGCTTCGGGGGAGTGATTCCCTGGCTAACCATCTGATTAATGGCTTCCAGTTGCTGTATG
  TGGGTGATGCCCTCTGGTATGAGGAGTCTGTCCTCACCACCATGGACATAATACATGATGGTTTTGGCTT
  CATGCTGGTCTTCGGAGATCTAGCTTGGGTACCATTCACCTACAGCCTGCAGGCCCAGTTCCTGTTGTAC
  CATCCACAGCCTCTGGGGTTGCCCATGGCCTTGCTCATCTGCCTCCTTAAGGCTATTGGTTACTACATGT
  TCCGAGGGGCCAACTCCCAGAAAAACACATTCAGGAAGAATCCTTCTGACCCCAGCGTGGCTGGTCTTGA
  GACCATCCCGACTGCCACGGGGAGGCAGCTGCTGGTGTCTGGGTGGTGGGGTATGGTTCGACACCCCAAC
  TACCTGGGAGATCTCATCATGGCTCTGGCATGGTCTTTGCCCTGTGGGCTATCCCATGGGTCCCTTCCCT
  CCAGGGCTATCCCATCTGCTGCCCTACTTCTACGTCCTCTACTTCACTGCATTGCTGGCGCACCGCGAGG
  CCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206271 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSREASQAPLEFGGPLGVAALLILLPATMFHLLLAARSGPARLLALPAYLPGLEELWSPWALLLLFIWL
  GLQVALYLLPARKVAEGLELKDKSRLRYPINGFQALVLTALLMGLGVSVGLPLGALPGMLLPLAFATTLT
  SFIFSLLLYAKALVAPASALAPGGNSGNSMYDFFLGRELNPRLGSFDFKYFCELRPGLIGWVFINLALLM
  QEAELRGSDSLANHLINGFQLLYVGDALWYEESVLTTMDIIHDGFGFMLVFGDLAWVPFTYSLQAQFLLY
  HPQPLGLPMALLICLLKAIGYYMFRGANSQKNTFRKNPSDPSVAGLETIPTATGRQLLVSGWWGMVRHPN
  YLGDLIMALAWSLPCGLSHGSLPSRAIPSAALLLRPLLHCIAGAPRGQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC014769
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC014769, AAH14769
• RefSeq Size: 1523 bp
• RefSeq ORF: 1199 bp
• Locus ID: 73166
• MW: 43.4 kDa