Kcnab1 (NM_010597) Mouse Tagged ORF Clone

CAT#: MR206299

Kcnab1 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, beta member 1 (Kcnab1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnab1
• Synonyms: Akr8a8; Kvbeta1.1; mKv(beta)1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206299 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGTCTCCATAGCCTGCACAGAGCACAATTTGAAGAGTCGAAATGGTGAGGACCGACTTCTGAGCA
  AGCAGAGCTCCAACGCCCCCAATGTGGTGAACGCAGCCCGGGCCAAATTCCGCACTGTCGCTATCATCGC
  TCGCAGCCTGGGGACCTTCACCCCTCAGCATCACATTTCTCTCAAAGAGTCCACCGCAAAGCAGACTGGC
  ATGAAATATAGGAATCTTGGGAAATCAGGACTCAGAGTTTCATGCTTGGGTCTTGGAACATGGGTGACAT
  TTGGAGGTCAAATCTCAGATGAGGTTGCTGAACGGCTGATGACAATTGCCTACGAAAGTGGAGTTAATCT
  CTTCGACACAGCTGAGGTCTATGCTGCTGGGAAGGCTGAGGTGATTCTGGGAAGCATCATCAAGAAGAAA
  GGCTGGAGGAGGTCCAGCTTGGTCATCACAACCAAACTCTACTGGGGTGGAAAAGCTGAGACAGAAAGGG
  GACTGTCAAGAAAGCACATCATTGAAGGACTGAAAGGCTCCCTCCAGAGGCTGCAACTGGAATACGTGGA
  TGTGGTCTTTGCAAATCGCCCAGACAGCAACACTCCCATGGAAGAAATCGTTCGAGCCATGACGCACGTG
  ATCAACCAAGGCATGGCCATGTACTGGGGCACCTCGAGGTGGAGCGCGATGGAGATCATGGAAGCCTACT
  CTGTCGCACGGCAGTTCAACATGATCCCGCCTGTCTGTGAGCAAGCTGAGTACCATCTTTTCCAGAGAGA
  GAAGGTGGAGGTCCAGCTGCCGGAGCTCTACCATAAAATAGGAGTTGGTGCAATGACATGGTCTCCACTT
  GCTTGTGGAATTATTTCAGGAAAATATGGAAATGGGGTGCCAGAAAGTTCTAGAGCTTCACTGAAGTGCT
  ACCAGTGGTTGAAGGAAAGAATCGTAAGTGAAGAAGGGAGAAAACAGCAAAACAAGCTGAAAGACCTCTC
  TCCAATCGCTGAGCGCCTGGGGTGCACGCTACCTCAGCTGGCTGTGGCGTGGTGCCTGAGAAATGAGGGT
  GTGAGTTCTGTGCTCCTGGGATCATCCACTCCGGAACAACTCATTGAAAACCTTGGTGCCATTCAGGTCC
  TCCCTAAGATGACATCTCACGTGGTGAACGAGATTGATAACATACTGCGCAACAAGCCCTACAGCAAAAA
  GGACTATAGATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206299 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQVSIACTEHNLKSRNGEDRLLSKQSSNAPNVVNAARAKFRTVAIIARSLGTFTPQHHISLKESTAKQTG
  MKYRNLGKSGLRVSCLGLGTWVTFGGQISDEVAERLMTIAYESGVNLFDTAEVYAAGKAEVILGSIIKKK
  GWRRSSLVITTKLYWGGKAETERGLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEIVRAMTHV
  INQGMAMYWGTSRWSAMEIMEAYSVARQFNMIPPVCEQAEYHLFQREKVEVQLPELYHKIGVGAMTWSPL
  ACGIISGKYGNGVPESSRASLKCYQWLKERIVSEEGRKQQNKLKDLSPIAERLGCTLPQLAVAWCLRNEG
  VSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEIDNILRNKPYSKKDYRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010597
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010597.1, NM_010597.2, NM_010597.3, NM_010597.4, NP_034727.2
• RefSeq Size: 3267 bp
• RefSeq ORF: 1206 bp
• Locus ID: 16497
• MW: 44.7 kDa