Ss18l1 (NM_178750) Mouse Tagged ORF Clone

CAT#: MR206309

Ss18l1 (Myc-DDK-tagged) - Mouse synovial sarcoma translocation gene on chromosome 18-like 1 (Ss18l1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ss18l1
• Synonyms: A230053O16Rik; CREST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206309 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTGGCCTTCGCGTCGGCGCGGCCGAGAGGCAAAGGGGAGGTCACTCAGCAGACCATCCAGAAGA
  TGCTGGATGAGAACCACCACCTGATCCAGTGCATCCTGGACTACCAGAGCAAGGGCAAGACCGCCGAGTG
  CACGCAGTACCAGCAGATCCTGCACCGGAACCTGGTCTACCTAGCCACCATAGCAGACTCCAATCAGAAC
  ATGCAGTCCCTGCTTCCCGCGCCTCCAACACAGAACATGAACCTCGGGCCCGGAGCACTGAGTCAGAGTG
  GTTCCAGCCAGGGCCTGCACCCCCAGGGCAGCCTCAGCGATACCGTCAGCACAGGCCTGCCCCCCGCCTC
  CCTCATGCAGGGCCAGATCGGTAACGGTCCAAACCACGTGTCCATGCAGCAGACGGCTCAGAGCACACTG
  CCCACAACCTCCATGAGCTTGTCAGGCAGTGGCCATGGTACTGGCCCTGGGTACAGCCACTCGGGGCCTA
  CCTCGCAGAGTGTCCCCATGCAAGGCCAAGGTGCCATCAGCAACTATGTGTCTCGGACCAACATCAACAT
  GCAGTCTAACCCAGTCTCCATGATGCACCAGCAGGCAGCCACGTCCCACTACAACTCAGCACAGGGTGGA
  AGCCAGCATTACCAGGGCCAGGCACCCATTGCCATGATGGGCCAGGGTGGCCAAGGAGGCAGCATGATGG
  GGCAGCGGCCCATGGCGCCCTACAGACCCTCCCAGCAAGGCTCTTCCCAGCAGTACCTGGGCCAAGAGGA
  GTACTACAGCGAACAGTACAGCCACAGCCAGGGCTCCGCAGAGCCCATGAGTCAACAGTACTACCCGGAT
  GGCCACGGTGACTACGCCTATCAGCAGTCGTCCTACACAGAGCAGAGCTACGACCGCTCCTTTGAGGATC
  CCACACAGCACTACTACGAGGGGGGAAACTCCCAGTACAGTCAGCAGCAGGCTGGGTACCAGCAGGGCAC
  AGCACAGCAGCAGACCTACTCCCAGCAACAGTATCCCAACCAGCAGAGCTACCCGGGGCAGCAGCAGGGC
  TATGGTCCTGCCCAGGGAGCCCCCTCACAGTACTCAAGCTACCAGCAAGGACAAGGTCAGCAGTATGGAA
  GCTACAGAACATCGCAGACGGGACCTTCTGCCCAGCAGCAGCGGCCTTACGGCTATGAACAGGGCCAGTA
  TGGAAATTACCAGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206309 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVAFASARPRGKGEVTQQTIQKMLDENHHLIQCILDYQSKGKTAECTQYQQILHRNLVYLATIADSNQN
  MQSLLPAPPTQNMNLGPGALSQSGSSQGLHPQGSLSDTVSTGLPPASLMQGQIGNGPNHVSMQQTAQSTL
  PTTSMSLSGSGHGTGPGYSHSGPTSQSVPMQGQGAISNYVSRTNINMQSNPVSMMHQQAATSHYNSAQGG
  SQHYQGQAPIAMMGQGGQGGSMMGQRPMAPYRPSQQGSSQQYLGQEEYYSEQYSHSQGSAEPMSQQYYPD
  GHGDYAYQQSSYTEQSYDRSFEDPTQHYYEGGNSQYSQQQAGYQQGTAQQQTYSQQQYPNQQSYPGQQQG
  YGPAQGAPSQYSSYQQGQGQQYGSYRTSQTGPSAQQQRPYGYEQGQYGNYQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178750
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178750.1, NM_178750.2, NM_178750.3, NM_178750.4, NM_178750.5, NP_848865.3
• RefSeq Size: 4395 bp
• RefSeq ORF: 1209 bp
• Locus ID: 269397
• MW: 43.7 kDa