Tacr1 (NM_009313) Mouse Tagged ORF Clone

CAT#: MR206405

Tacr1 (Myc-DDK-tagged) - Mouse tachykinin receptor 1 (Tacr1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tacr1
• Synonyms: Nk1r; Spr; Tac1r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206405 representing NM_009313
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAACGTCCTTCCTGTGGACTCTGATCTCTTCCCCAACACCTCCACCAACACTTCTGAGTCTAACC
  AGTTTGTGCAACCTACCTGGCAAATTGTCCTTTGGGCAGCCGCCTATACGGTCATCGTGGTGACTTCCGT
  GGTGGGCAACGTAGTGGTGATATGGATCATTTTGGCCCACAAGAGAATGAGGACAGTGACCAATTATTTC
  CTGGTGAACCTGGCCTTCGCTGAGGCCTGCATGGCTGCATTCAATACAGTGGTGAACTTCACCTACGCAG
  TCCACAACGTGTGGTACTACGGCCTCTTTTACTGCAAGTTTCACAACTTCTTCCCCATTGCTGCTCTCTT
  CGCCAGTATCTACTCCATGACAGCTGTGGCCTTTGACAGATACATGGCCATCATCCACCCTCTTCAGCCC
  CGGCTGTCGGCCACTGCTACCAAAGTGGTCATCTTTGTCATCTGGGTCCTGGCTCTCCTGCTGGCCTTCC
  CACAGGGCTACTACTCCACCACAGAGACCATGCCCAGCAGAGTAGTGTGCATGATTGAGTGGCCAGAACA
  TCCCAACAGGACTTACGAGAAAGCGTACCACATCTGTGTGACTGTGCTGATCTACTTCCTGCCTCTGCTG
  GTGATTGGCTATGCATACACTGTGGTAGGGATTACACTGTGGGCCAGTGAGATCCCCGGTGACTCCTCTG
  ACCGTTACCATGAGCAAGTCTCTGCCAAGCGCAAGGTGGTCAAAATGATGATCGTGGTTGTGTGTACCTT
  CGCCATCTGCTGGCTGCCCTTCCACATCTTCTTCCTCCTGCCCTACATCAACCCAGATCTCTACCTTAAG
  AAGTTCATCCAGCAGGTCTACCTGGCCAGCATGTGGCTGGCCATGAGTTCTACCATGTACAACCCCATCA
  TCTACTGCTGCCTCAATGACAGGTTCCGTCTGGGCTTCAAGCATGCCTTTCGCTGCTGCCCTTTCATCAG
  TGCTGGTGATTATGAGGGGCTGGAAATGAAATCCACCCGATACCTCCAGACCCAGAGCAGCGTGTACAAG
  GTCAGCCGCCTGGAGACCACCATCTCCACTGTGGTGGGAGCCCATGAAGATGAGCCAGAGGAAGGTCCCA
  AGGCCACACCTTCATCCTTGGATCTCACCTCCAATGGCTCCTCTCGAAGCAACTCGAAGACCATGACAGA
  AAGCTCCAGCTTCTACTCTAACATGCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206405 representing NM_009313
  Red=Cloning site Green=Tags(s)
  
  MDNVLPVDSDLFPNTSTNTSESNQFVQPTWQIVLWAAAYTVIVVTSVVGNVVVIWIILAHKRMRTVTNYF
  LVNLAFAEACMAAFNTVVNFTYAVHNVWYYGLFYCKFHNFFPIAALFASIYSMTAVAFDRYMAIIHPLQP
  RLSATATKVVIFVIWVLALLLAFPQGYYSTTETMPSRVVCMIEWPEHPNRTYEKAYHICVTVLIYFLPLL
  VIGYAYTVVGITLWASEIPGDSSDRYHEQVSAKRKVVKMMIVVVCTFAICWLPFHIFFLLPYINPDLYLK
  KFIQQVYLASMWLAMSSTMYNPIIYCCLNDRFRLGFKHAFRCCPFISAGDYEGLEMKSTRYLQTQSSVYK
  VSRLETTISTVVGAHEDEPEEGPKATPSSLDLTSNGSSRSNSKTMTESSSFYSNMLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009313
• ORF Size: 1221 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009313.1, NM_009313.2, NM_009313.3, NM_009313.4, NM_009313.5, NP_033339.2
• RefSeq Size: 5021 bp
• RefSeq ORF: 1224 bp
• Locus ID: 21336
• MW: 46.8 kDa
• Gene Summary: This gene encodes the receptor for the tachykinin, substance P, also referred to as neurokinin 1. This gene belongs to a gene family of tachykinin receptors which are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This receptor has been associated with nitric oxide formation, and it has been localized to cholinergic and nitrergic neurons as well as on smooth muscle cells. [provided by RefSeq, Mar 2010]