Spred2 (NM_033523) Mouse Tagged ORF Clone

CAT#: MR206449

Spred2 (Myc-DDK-tagged) - Mouse sprouty-related, EVH1 domain containing 2 (Spred2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spred2
• Synonyms: C79158
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206449 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAAGAAACACACCCGGACGATGACAGCTATATTGTGCGTGTCAAGGCTGTGGTTATGACCAGAG
  ATGACTCCAGCGGGGGATGGTTCCCACAGGAAGGAGGCGGGATCAGTCGCGTCGGCGTGTGTAAGGTCAT
  GCACCCTGAAGGCAACGGACGAAGCGGCTTTCTCATCCATGGCGAGCGACAGAAAGACAAACTGGTGGTA
  TTGGAATGCTATGTCAGAAAGGACTTGGTCTACACCAAAGCCAATCCGACGTTTCATCATTGGAAGGTTG
  ATAACAGGAAGTTTGGACTTACTTTCCAAAGTCCTGCAGATGCACGAGCCTTTGACAGGGGCGTGAGAAA
  AGCCATTGAAGACCTTATAGAAGGTTCAACGACCTCCTCTTCCACTCTCCATAACGAAGCTGAGCTCGGA
  GACGATGACGTTTTCACGACAGCTACGGACAGTTCTTCTAATTCCTCGCAGAAGAGGGAGCCGACTACGA
  GGACAATCTCCTCCCCCACGTCCTGTGAGCACCGGAAGATTTATACCCTTGACCCATACCCCATGGACCA
  TTACCACCCTGACCAGCGGTTGCCGCGGTCCTACCCCCAGGTCACCTTCCCAGAAGATGATGAAGAAATT
  GTACGCATCAACCCCCGAGAGAAGATCTGGATGACCGGTTATGAAGACTACCGGCACGCGCCGGTTCGCG
  GCAAATACTTAGACACCACAGAAGACGCGGACTCCTACGTGCGCTTCGCCAAGGGCGAAGTCCCCAAACA
  CGAATATACCTATCCCTATGTTGATTCTTCGGACTTCGGCTTCGGGGAGGATCCCAAAGGTAGTGTGATC
  AAGACACAGCCGCCCAGGGCCAAGTCCCGTCGGCGGAAGGAGAACGGCGAACGGTCGCGGTGTGTGTACT
  GCAGGGATATGTTTAATCACGAAGAGAACCGAAGGGGCCACTGCCAAGACGCGCCCGACGCCGTGAGAAC
  TTGCATTCGCCGGGTGAGCTGTATGTGGTGCGCGGACAGCATGCTGTACCACTGTATGTCCGACCCCGAG
  GGAGACTACACTGACCCTTGTTCGTGTGACACAAGCGATGAGAAGTTTTGCCTCCGGTGGATGGCTCTAA
  TTGCCTTGTCTTTCCTGGCCCCTTGTATGTGCTGTTACCTGCCCCTCCGGGCCTGCCACCGCTGTGGAGT
  GATGTGCAGGTGCTGTGGTGGGAAGCACAAAGCCGCCGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206449 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIHGERQKDKLVV
  LECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIEDLIEGSTTSSSTLHNEAELG
  DDDVFTTATDSSSNSSQKREPTTRTISSPTSCEHRKIYTLDPYPMDHYHPDQRLPRSYPQVTFPEDDEEI
  VRINPREKIWMTGYEDYRHAPVRGKYLDTTEDADSYVRFAKGEVPKHEYTYPYVDSSDFGFGEDPKGSVI
  KTQPPRAKSRRRKENGERSRCVYCRDMFNHEENRRGHCQDAPDAVRTCIRRVSCMWCADSMLYHCMSDPE
  GDYTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACHRCGVMCRCCGGKHKAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033523
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033523.1, NM_033523.2, NM_033523.3, NM_033523.4, NP_277058.1
• RefSeq Size: 2872 bp
• RefSeq ORF: 1233 bp
• Locus ID: 114716
• MW: 46.8 kDa