Csnk1d (NM_139059) Mouse Tagged ORF Clone

CAT#: MR206546

Csnk1d (Myc-DDK-tagged) - Mouse casein kinase 1, delta (Csnk1d), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Csnk1d
• Synonyms: 1200006A05Rik; AA409348; D930010H05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206546 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAGGGTCGGGAACAGGTACCGACTGGGCCGCAAGATCGGCAGCGGCTCCTTCGGAGACATCT
  ATCTCGGTACGGACATTGCTGCGGGAGAAGAAGTTGCCATCAAGCTTGAATGTGTCAAAACCAAACATCC
  TCAGCTCCACATTGAGAGCAAGATCTACAAAATGATGCAGGGAGGAGTGGGCATCCCTACCATCAGATGG
  TGTGGGGCTGAGGGGGACTACAATGTCATGGTGATGGAGCTACTGGGACCCAGCCTGGAAGACCTGTTCA
  ACTTCTGTTCAAGGAAGTTTAGTCTCAAAACTGTTCTGTTGCTTGCTGACCAAATGATAAGTCGTATTGA
  GTACATTCATTCGAAGAATTTTATCCACCGAGATGTGAAGCCAGATAACTTCCTCATGGGGCTGGGAAAG
  AAAGGCAACCTGGTCTACATCATTGACTTTGGGCTGGCCAAGAAGTATCGGGATGCACGCACCCACCAGC
  ATATCCCCTATCGAGAGAACAAGAACCTCACAGGGACAGCACGCTATGCCTCCATCAACACGCACCTTGG
  CATTGAACAATCTCGAAGGGATGACTTGGAGTCTCTGGGGTACGTGCTGATGTACTTCAACCTGGGCTCT
  CTCCCCTGGCAGGGGCTGAAGGCCGCCACCAAGAGGCAGAAGTATGAGAGGATCAGTGAGAAGAAGATGT
  CCACTCCCATTGAAGTGCTGTGCAAGGGCTATCCTTCTGAATTTGCCACATACCTGAATTTCTGCCGTTC
  CTTACGTTTTGATGACAAACCTGACTACTCCTACCTGAGACAGCTCTTCAGAAATCTGTTCCATCGCCAA
  GGCTTCTCCTACGACTATGTGTTCGACTGGAACATGCTCAAATTTGGTGCCAGCCGGGCTGCAGATGATG
  CTGAGCGGGAACGCCGGGATCGAGAAGAACGATTAAGACACTCCCGGAATCCAGCCACTCGTGGCCTCCC
  TTCTACAGCTTCCGGCCGTCTGCGGGGAACCCAGGAAGTGGCTCCCCCAACGCCCCTTACCCCTACCTCA
  CACACGGCCAACACCTCTCCGAGGCCTGTCTCTGGCATGGAACGAGAACGGAAAGTGAGTATGCGGCTGC
  ACCGTGGGGCCCCAGTCAACGTCTCCTCATCTGATCTCACGGGCCGACAAGATACCTCTCGCATGTCCAC
  CTCACAGATTCCCGGTCGGGTGGCTTCCAGTGGTCTTCAGTCTGTCGTGCACCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206546 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELRVGNRYRLGRKIGSGSFGDIYLGTDIAAGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIRW
  CGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGK
  KGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGS
  LPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQ
  GFSYDYVFDWNMLKFGASRAADDAERERRDREERLRHSRNPATRGLPSTASGRLRGTQEVAPPTPLTPTS
  HTANTSPRPVSGMERERKVSMRLHRGAPVNVSSSDLTGRQDTSRMSTSQIPGRVASSGLQSVVHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139059
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139059.1, NM_139059.2, NP_620690.1
• RefSeq Size: 3686 bp
• RefSeq ORF: 1248 bp
• Locus ID: 104318
• MW: 47.3 kDa
• Gene Summary: This gene encodes a member of the casein kinase I (CKI) family of serine/threonine protein kinases. A highly similar human protein regulates an array of cellular processes by influencing the Wnt and hedgehog signaling pathways. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]