Wnt10a (NM_009518) Mouse Tagged ORF Clone

CAT#: MR206595

Wnt10a (Myc-DDK-tagged) - Mouse wingless related MMTV integration site 10a (Wnt10a)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wnt10a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206595 representing NM_009518
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCGCCCACCCTCGCCCCTGGCTGCGGCTCCCACAAGGGCCCCAGCCGCGGCCTGAGTTCTGGG
  CGCTCCTGTTCTTCCTACTGCTGCTGGCTGCCGCTGTGCCCAGGTCAGCACCCAACGACATCCTGGGCCT
  CCGCCTACCCCCAGAGCCCGTGCTCAACGCCAACACAGTGTGCCTGACATTGCCCGGCCTGAGCCGGCGG
  CAGATGGAGGTGTGTGTGCGTCACCCTGACGTGGCCGCCTCTGCTATCCAGGGCATCCAGATCGCCATCC
  ATGAGTGCCAGCATCAGTTCCGGGACCAGCGCTGGAACTGCTCCAGCCTGGAGACTCGGAACAAAGTCCC
  CTACGAGAGCCCCATCTTCAGCCGAGGTTTTCGAGAGAGTGCTTTCGCCTACGCCATAGCAGCTGCCGGG
  GTGGTGCACGCAGTGTCCAACGCGTGCGCTCTGGGTAAACTGAAGGCTTGCGGTTGCGACGCCTCCAGAC
  GTGGGGACGAAGAAGCTTTCCGTCGGAAGCTGCACCGCTTGCAGCTGGACGCGCTGCAGCGCGGAAAGGG
  CTTGAGCCACGGGGTCCCTGAACACCCGGCCATACTTCCTGCCAGCCCAGGTCTGCAGGACTCCTGGGAG
  TGGGGTGGCTGCAGTCCGGATGTGGGCTTCGGAGAACGCTTCTCTAAGGACTTTCTGGACTCCCGAGAGC
  CTCACAGAGACATCCATGCTCGAATGAGACTCCACAACAACCGTGTGGGCCGGCAGGCGGTGATGGAGAA
  CATGCGGCGTAAGTGCAAATGCCACGGCACCTCAGGCAGCTGCCAGCTCAAGACCTGCTGGCAGGTGACG
  CCTGAGTTCCGCACAGTAGGGGCGCTGCTGCGCAACCGCTTCCACCGCGCCACGCTCATCCGGCCGCACA
  ACCGCAACGGTGGCCAGCTGGAGCCCGGCCCCGCGGGAGCACCCTCGCCAGCACCGGGCACTCCAGGGCT
  GCGCCGCAGGGCCAGCCACTCCGACCTGGTCTACTTTGAGAAATCTCCCGACTTCTGTGAGCGCGAGCCG
  CGCCTGGACTCGGCAGGCACTGTGGGCCGCCTGTGCAATAAGAGCAGCACGGGTCCCGATGGCTGCGGCA
  GCATGTGCTGTGGCCGCGGCCACAACATTCTGCGCCAGACGCGCAGCGAGCGCTGCCACTGCCGGTTCCA
  CTGGTGCTGCTTCGTGGTCTGCGAAGAATGCCGCATCACCGAGTGGGTCAGCGTCTGCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206595 representing NM_009518
  Red=Cloning site Green=Tags(s)
  
  MGSAHPRPWLRLPQGPQPRPEFWALLFFLLLLAAAVPRSAPNDILGLRLPPEPVLNANTVCLTLPGLSRR
  QMEVCVRHPDVAASAIQGIQIAIHECQHQFRDQRWNCSSLETRNKVPYESPIFSRGFRESAFAYAIAAAG
  VVHAVSNACALGKLKACGCDASRRGDEEAFRRKLHRLQLDALQRGKGLSHGVPEHPAILPASPGLQDSWE
  WGGCSPDVGFGERFSKDFLDSREPHRDIHARMRLHNNRVGRQAVMENMRRKCKCHGTSGSCQLKTCWQVT
  PEFRTVGALLRNRFHRATLIRPHNRNGGQLEPGPAGAPSPAPGTPGLRRRASHSDLVYFEKSPDFCEREP
  RLDSAGTVGRLCNKSSTGPDGCGSMCCGRGHNILRQTRSERCHCRFHWCCFVVCEECRITEWVSVCK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009518
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009518.1, NM_009518.2, NP_033544.1
• RefSeq Size: 2487
• RefSeq ORF: 1254
• Locus ID: 22409
• MW: 46.9 kDa
• Gene Summary: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. [UniProtKB/Swiss-Prot Function]