Serpinf1 (NM_011340) Mouse Tagged ORF Clone

CAT#: MR206610

Serpinf1 (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade F, member 1 (Serpinf1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Serpinf1
• Synonyms: AI195227; EPC-1; Pedf; Pedfl; Sdf3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206610 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCCCTGGTGCTACTCCTCTGGACTGGAGCCCTGCTCGGGCACGGCAGCAGCCAGAACGTCCCCA
  GCAGCTCTGAGGGCTCCCCAGTCCCGGACAGCACGGGCGAGCCCGTGGAGGAGGAGGACCCCTTCTTCAA
  GGTCCCTGTGAACAAGCTGGCAGCAGCTGTCTCCAACTTCGGCTACGATCTGTACCGCCTGAGATCCAGT
  GCCAGCCCAACGGGCAACGTCCTGCTGTCTCCACTCAGCGTGGCCACGGCCCTCTCTGCCCTTTCTCTGG
  GAGCTGAACATCGAACAGAGTCTGTCATTCACCGGGCTCTCTACTACGACCTGATCACCAACCCTGACAT
  CCACAGCACCTACAAGGAGCTCCTTGCCTCTGTTACTGCCCCTGAGAAGAACCTCAAGAGTGCTTCCAGA
  ATTGTGTTTGAGAGGAAACTTCGAGTCAAATCCAGCTTTGTTGCCCCTCTGGAGAAGTCCTATGGGACCA
  GGCCCCGGATCCTCACGGGCAACCCTCGAGTAGACCTTCAGGAGATTAACAACTGGGTGCAGGCCCAGAT
  GAAAGGGAAGATTGCCCGGTCCACGAGGGAAATGCCCAGTGCCCTCAGCATCCTTCTCCTTGGCGTGGCT
  TACTTCAAGGGGCAGTGGGTAACCAAGTTTGACTCGAGAAAGACGACCCTCCAGGATTTTCATTTGGACG
  AGGACAGGACCGTGAGAGTCCCCATGATGTCAGATCCTAAGGCCATCTTACGATACGGCTTGGACTCTGA
  TCTCAACTGCAAGATTGCCCAGCTGCCCTTGACAGGAAGTATGAGCATCATCTTCTTCCTGCCCCTGACC
  GTGACCCAGAACTTGACCATGATAGAAGAGAGCCTCACCTCTGAGTTCATTCATGACATCGACCGAGAAC
  TGAAGACTATCCAAGCTGTGCTGACTGTCCCCAAGCTGAAGCTGAGCTTCGAAGGCGAACTTACCAAGTC
  TCTGCAGGACATGAAGCTACAGTCGTTGTTTGAATCACCCGACTTCAGCAAGATTACTGGCAAACCCGTG
  AAGCTCACCCAAGTGGAACACAGGGCTGCTTTCGAGTGGAATGAAGAGGGGGCAGGAAGCAGCCCCAGCC
  CAGGCCTCCAGCCCGTCCGCCTCACCTTCCCGCTAGACTATCACCTTAACCAACCTTTCCTCTTTGTTCT
  GAGGGACACGGACACGGGGGCCCTCCTCTTCATAGGCAGAATCCTGGACCCCAGTAGTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206610 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQALVLLLWTGALLGHGSSQNVPSSSEGSPVPDSTGEPVEEEDPFFKVPVNKLAAAVSNFGYDLYRLRSS
  ASPTGNVLLSPLSVATALSALSLGAEHRTESVIHRALYYDLITNPDIHSTYKELLASVTAPEKNLKSASR
  IVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVA
  YFKGQWVTKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLT
  VTQNLTMIEESLTSEFIHDIDRELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPV
  KLTQVEHRAAFEWNEEGAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011340
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011340.1, NM_011340.2, NM_011340.3, NP_035470.3
• RefSeq Size: 1497 bp
• RefSeq ORF: 1254 bp
• Locus ID: 20317
• MW: 46.2 kDa