Acss1 (BC030930) Mouse Tagged ORF Clone

CAT#: MR206638

Acss1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase short-chain family member 1 (cDNA clone MGC:31687 IMAGE:4911679)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acss1
• Synonyms: AceCS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206638 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGAGGCCCCTGTTTGCACCCCTGAGAGCATGAGCAGTGAAGACATGCTCTTTATGCTCTACA
  CCTCAGGGAGCACCGGGACACCCAAGGGACTCGTTCATACACAGGCAGGCTATCTACTGTATGCCGCCAT
  GACGCACAAGCTCGTGTTTGACTACCAGCCAGGTGATGTCTTTGGCTGTGTGGCTGACATCGGTTGTATC
  ACAGGACACAGCTATGTGGTGTATGGACCCCTCTGCAATGGAGCTACCACAGTCCTTTTTGAGAGCACCC
  CAGTTTACCCTGATGCTGGTCGTTACTGGGAGACAGTGCAGAGGCTAAAGATCAACCAGTTCTATGGTGC
  CCCGACAGCTGTCCGGCTGCTGCTGAAGTATGGGGATGCCTGGGTGAAAAAGTATGACCGCTCTTCCCTG
  CGCACACTGGGGTCAGTGGGAGAGCCTATCAACCACGAAGCCTGGGAGTGGCTCCACAAAGTCGTGGGTG
  ATGGCAGATGTACACTGGTGGACACTTGGTGGCAAACGGAAACTGGAGGCATCTGCATTGCACCACGGCC
  CTCGGAAGATGGGGCAGAGATCCTCCCGGGCATGGCCATGAGGCCGTTTTTTGGCATCGTTCCTGTACTC
  ATGGATGAGAAGGGCAATGTTTTGGAGGGTGGAGATGTCTCTGGGGCCTTGTGTATTTCCCAAGCTTGGC
  CAGGCATGGCAAGGACCATCTATGGTGACCACCAGAGGTTCGTAGATGCCTACTTCAGAGCGTACCCAGG
  TTATTACTTCACTGGAGACGGAGCTCACCGGACAGAGGGTGGCTATTACCAGATCACGGGGCGCATGGAT
  GATGTCATCAATATCAGTGGTCATCGCCTGGGGACTGCAGAGATTGAGGATGCAATGGCTGACCATCCCG
  CTGTTCCAGAGACTGCTGTCATTGGGTACCCTCATGATATCAAAGGAGAAGCTGCATTTGCCTTCATTGT
  GCTGAAAGATAACATCAGTGATGAGAACATGGTAGTGAATGAACTCAAATTGTCGGTGGCCACCAAGATC
  GCCAAGTATGCTGTGCCTGACCAGATCCTGGTGGTGAAGCGTCTCCCCAAAACCAGATCTGGGAAAGTGA
  TGAGGAGACTACTGAGGAAGATCATCACCAGCAGGGGACAGGATCTAGGGGACACCACTACCCTGGAGGA
  CCCCAGCGTCATCACAGAAATCTTGAGTGCCTTCCAGAAGTATGAAGAGCAGCGGGCTGCTACCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206638 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKEAPVCTPESMSSEDMLFMLYTSGSTGTPKGLVHTQAGYLLYAAMTHKLVFDYQPGDVFGCVADIGCI
  TGHSYVVYGPLCNGATTVLFESTPVYPDAGRYWETVQRLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSL
  RTLGSVGEPINHEAWEWLHKVVGDGRCTLVDTWWQTETGGICIAPRPSEDGAEILPGMAMRPFFGIVPVL
  MDEKGNVLEGGDVSGALCISQAWPGMARTIYGDHQRFVDAYFRAYPGYYFTGDGAHRTEGGYYQITGRMD
  DVINISGHRLGTAEIEDAMADHPAVPETAVIGYPHDIKGEAAFAFIVLKDNISDENMVVNELKLSVATKI
  AKYAVPDQILVVKRLPKTRSGKVMRRLLRKIITSRGQDLGDTTTLEDPSVITEILSAFQKYEEQRAATN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC030930
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030930, AAH30930
• RefSeq Size: 2783 bp
• RefSeq ORF: 1259 bp
• Locus ID: 68738
• MW: 46.2 kDa