Ckmt2 (NM_198415) Mouse Tagged ORF Clone

CAT#: MR206653

Ckmt2 (Myc-DDK-tagged) - Mouse creatine kinase, mitochondrial 2 (Ckmt2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ckmt2
• Synonyms: 2300008A19Rik; ScCKmit
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206653 representing NM_198415.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCAGTGCCTTCTCAAAGTTGCTAACTGGCCGAAATGCATCTCTGCTGTTTACTACACTGGGAACC
  AGTGCCCTGACCACTGGTTACCTGCTGAATAGGCAGAAGGTATCTGCTGATGCCCGGGAACAGCACAAG
  CTCTTTCCCCCAAGTGCGGACTACCCTGACCTTCGAAAACATAACAACTGCATGGCTGAGTGCCTCACC
  CCCACCATCTATGCCAAACTCCGAAACAAGATGACACCCAGTGGCTATACCCTGGACCAGTGCATCCAA
  ACTGGAGTGGACAACCCCGGCCACCCCTTCATTAAGACTGTGGGCATGGTGGCTGGTGATGAGGAGTCG
  TATGAGGTATTTGCTGACCTTTTTGATCCTGTCATCAAACTAAGGCACAATGGCTATGACCCTAGGGTG
  ATGAAGCATCCTACGGACCTGGATGCATCCAAGATTACTCACGGGCAGTTTGATGAGCGCTATGTGTTG
  TCCTCTCGGGTACGCACTGGCCGAAGCATCCGCGGGCTGAGCCTGCCTCCAGCCTGCTCCCGAGCAGAG
  AGAAGGGAGGTGGAGAATGTGGCCATTACTGCCCTGGAGGGCCTGAAGGGCGATCTGGCTGGCCGCTAC
  TACAAGCTGTCTGAGATGACGGAGCAGGATCAGCAACGTCTCATCGATGACCACTTTCTGTTTGATAAG
  CCAGTGTCCCCTTTACTAACATGTGCTGGAATGGCCCGTGACTGGCCTGATGCCAGGGGAATCTGGCAT
  AATTATGACAAGACATTTCTCATCTGGATAAATGAGGAAGACCACACCAGGGTGATCTCAATGGAAAAG
  GGAGGCAATATGAAACGCGTATTTGAGAGATTCTGTCGTGGACTAAAGGAAGTGGAACGATTAATCCAG
  GAACGAGGCTGGGAGTTCATGTGGAATGAGCGTCTGGGATACATTTTGACTTGCCCCTCGAACCTCGGA
  ACTGGATTGCGAGCTGGTGTCCACGTTAGGATCCCGAAGCTCAGCAAGGATCCACGCTTTTCTAAGATC
  TTGGAGAACCTGCGGCTCCAAAAGCGTGGCACAGGTGGTGTGGACACTGCTGCAGTGGCAGATGTGTAC
  GATATTTCCAACATAGATCGGATCGGCAGATCAGAGGTTGAGCTTGTTCAGATTGTTATTGACGGAGTC
  AACTACCTGGTGGATTGTGAAAAGAAGCTGGAGAGAGGCCAAGACATCAAGGTGCCCCCTCCTCTGCCT
  CAGTTTGGCAGGAAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR206653
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASAFSKLLTGRNASLLFTTLGTSALTTGYLLNRQKVSADAREQHKLFPPSADYPDLRKHNNCMAECLT
  PTIYAKLRNKMTPSGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRV
  MKHPTDLDASKITHGQFDERYVLSSRVRTGRSIRGLSLPPACSRAERREVENVAITALEGLKGDLAGRY
  YKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEK
  GGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKI
  LENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLP
  QFGRK
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using MR206653 also available, TP506653
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198415
• ORF Size: 1257 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198415.3
• RefSeq Size: 1516 bp
• RefSeq ORF: 1260 bp
• Locus ID: 76722
• UniProt ID: Q6P8J7
• Cytogenetics: 13 C3
• MW: 47.5 kDa
• Gene Summary: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (By similarity).[UniProtKB/Swiss-Prot Function]