Cnp (NM_001146318) Mouse Tagged ORF Clone

CAT#: MR206670

Cnp (Myc-DDK-tagged) - Mouse 2',3'-cyclic nucleotide 3' phosphodiesterase (Cnp), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnp
• Synonyms: Cnp-1; Cnp1; CNPase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206670 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACAAGCTTTACCCGCAAAAGCCACACATTCCTGCCCAAGCTCTTCTTCAGGAAAATGTCATCCT
  CAGGAGCAAAGGAGAAGCCAGAGCTGCAGTTCCCTTTCCTTCAAGATGAGGACACGGTGGCTACCCTCCA
  CGAGTGCAAGACGCTATTCATCCTGCGCGGCCTGCCGGGCAGCGGCAAGTCCACACTGGCTCGGCTCATC
  CTGGAGAAGTACCACGACGGCACCAAGATGGTGTCCGCTGATGCTTACAAGATCATCCCTGGCTCTCGGG
  CAGACTTCTCCGAGGCGTACAAGCGTCTAGACGAGGACCTGGCTGGCTACTGCCGCCGGGACATCAGGGT
  TCTTGTGCTTGATGATACCAACCACGAGCGGGAGCGGCTGGATCAGCTTTTTGAAATGGCAGACCAGTAT
  CAGTACCAGGTGGTGCTGGTGGAGCCCAAGACAGCGTGGCGACTAGACTGTGCCCAGCTCAAGGAGAAGA
  ACCAATGGCAGCTGTCGGCCGATGACCTGAAGAAGCTGAAGCCCGGGCTGGAGAAGGACTTTCTGCCACT
  CTACTTTGGCTGGTTCCTGACCAAAAAGAGTTCTGAGACCCTCCGAAAAGCTGGCCAGGTCTTTCTGGAG
  GAGCTGGGGAATCACAAGGCCTTCAAGAAAGAGCTTCGACACTTTATTTCTGGAGATGAACCCAAGGAGA
  AGCTTGAGCTGGTCAGCTACTTTGGAAAGAGACCTCCAGGTGTGCTGCACTGTACAACCAAATTCTGTGA
  CTACGGGAAGGCCGCTGGGGCAGAAGAATACGCCCAGCAGGAGGTGGTGAAGAGATCGTATGGCAAGGCC
  TTCAAACTGTCCATCTCTGCTCTCTTTGTGACACCCAAGACAGCTGGGGCCCAGGTGGTGCTGACCGATC
  AGGAGCTGCAGTTGTGGCCCAGTGATCTGGACAAGCCATCTGCCTCCGAGGGCCTGCCCCCAGGGAGCCG
  AGCTCACGTCACCCTAGGCTGTGCGGCCGACGTGCAGCCAGTGCAGACGGGTCTTGACCTCTTAGATATT
  TTACAACAGGTGAAGGGGGGCAGCCAAGGTGAGGCGGTGGGTGAGCTCCCCCGGGGCAAGCTCTATTCCC
  TGGGCAAAGGGCGATGGATGCTGAGCCTGACTAAGAAGATGGAGGTCAAGGCCATCTTCACGGGGTACTA
  TGGGAAGGGCAAACCTGTGCCCATACATGGCAGCCGGAAGGGGGGTGCCATGCAGATCTGCACCATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206670 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNTSFTRKSHTFLPKLFFRKMSSSGAKEKPELQFPFLQDEDTVATLHECKTLFILRGLPGSGKSTLARLI
  LEKYHDGTKMVSADAYKIIPGSRADFSEAYKRLDEDLAGYCRRDIRVLVLDDTNHERERLDQLFEMADQY
  QYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLE
  ELGNHKAFKKELRHFISGDEPKEKLELVSYFGKRPPGVLHCTTKFCDYGKAAGAEEYAQQEVVKRSYGKA
  FKLSISALFVTPKTAGAQVVLTDQELQLWPSDLDKPSASEGLPPGSRAHVTLGCAADVQPVQTGLDLLDI
  LQQVKGGSQGEAVGELPRGKLYSLGKGRWMLSLTKKMEVKAIFTGYYGKGKPVPIHGSRKGGAMQICTII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146318
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2548 bp
• RefSeq ORF: 1203 bp
• Locus ID: 12799
• MW: 47.1 kDa