Nfib (NM_008687) Mouse Tagged ORF Clone

CAT#: MR206682

Nfib (Myc-DDK-tagged) - Mouse nuclear factor I/B (Nfib), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfib
• Synonyms: 6720429L07Rik; CTF; E030026I10Rik; NF-I/B; NF1-B; NFI-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206682 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTCCACCCATTTATTGAGGCACTTCTTCCTCACG
  TCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAA
  ACGAATGTCGAAGGATGAAGAAAGGGCAGTCAAAGACGAGCTGCTCAGTGAGAAGCCCGAAATCAAGCAG
  AAGTGGGCATCCAGGCTCCTGGCCAAACTGCGCAAAGATATCCGCCAGGAGTACCGGGAGGACTTTGTGC
  TTACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCAGACCAGAAGGGTAAGATTAGGAG
  GATCGACTGCCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC
  ATCCCTTTGGAGAGTACGGATGGAGAGCGACTCATGAAGTCCCCGCACTGCACAAACCCAGCACTTTGTG
  TTCAGCCACACCACATCACAGTATCAGTTAAGGAGCTTGACTTGTTTTTGGCATACTACGTGCAGGAGCA
  AGATTCTGGACAATCAGGAAGTCCAAGCCACAGTGATCCTGCCAAGAATCCTCCAGGGTACCTCGAGGAC
  AGCTTTGTAAAATCCGGAGTCTTCAATGTATCAGAGCTTGTGAGAGTATCCAGAACACCCATAACCCAGG
  GAACTGGAGTCAACTTCCCAATCGGAGAAATTCCCAGCCAACCATACTATCATGACATGAACTCTGGTGT
  GAACCTGCAGAGGTCGCTGTCTTCTCCACCGAGCAGCAAAAGACCCAAAACTATATCTATAGATGAAAAT
  ATGGAGCCAAGTCCTACAGGAGACTTTTACCCCTCTCCAAATTCACCAGCTGCTGGAAGTCGAACATGGC
  ATGAACGAGATCAAGATATGTCTTCTCCAACTACAATGAAGAAGCCTGAGAAGCCACTGTTTAGCTCTAC
  ATCTCCACAGGATTCTTCCCCAAGATTGAGCACTTTCCCCCAGCACCATCATCCCGGAATACCTGGAGTC
  GCGCACAGTGTCATCTCAACTCGAACTCCACCTCCGCCCTCACCGTTGCCATTTCCGACGCAAGCTATCC
  TTCCTCCGGCACCTTCCAGCTACTTCTCTCATCCAACAATCAGATATCCTCCTCACCTGAATCCTCAGGA
  TACTCTGAAGAACTACGTACCTTCTTATGACCCATCCAGTCCTCAAACGAGCCAGTCCTGGTACCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206682 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQ
  KWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKG
  IPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHSDPAKNPPGYLED
  SFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDEN
  MEPSPTGDFYPSPNSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSTSPQDSSPRLSTFPQHHHPGIPGV
  AHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQSWYLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008687
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008687.1, NM_008687.2, NM_008687.3, NM_008687.4, NM_008687.5, NM_008687.6, NP_032713.2
• RefSeq Size: 8932 bp
• RefSeq ORF: 1263 bp
• Locus ID: 18028
• MW: 47.5 kDa
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProtKB/Swiss-Prot Function]