Pus1 (NM_001025562) Mouse Tagged ORF Clone

CAT#: MR206726

  • TrueORF®

Pus1 (Myc-DDK-tagged) - Mouse pseudouridine synthase 1 (Pus1), transcript variant 3


  "NM_001025562" in other vectors (3)


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Reconstitution Protocol

USD 450.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Pus1
Synonyms A730013B20Rik; MPUS1; mPus1p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTTCCCCCGCCTGTGGGCCGCACTGCTGAGAAACTGGGGACGGTGGACGGCGCGCCCAGGCCCGC
GCGTGCCCGGCCTGCCGCCTATGGCTGGAAACAAGGTGCCACCAGCGCTGGCCTCACACCAACCGGACAG
GAAGGGCCGAGGTGGCTGGGTCTGGGAGGAGACGGAGCATCCTGCAAAGAGGGTCAAGGGCGGCGAGGAT
GAAGAGCCCCCACGCAAGCTGCCAAAGCGAAAAATTGTGCTGCTCATGGCCTACTCGGGCAAGGGCTACC
ACGGCATGCAGAGGAATCTGGGGTCCTCCCAGTTCAGGACCATCGAAGATGACTTGGTATCGGCCTTAGT
CCAGGCAGGTTGTATCCCTGAAAACCATGGCACAGATATGAGGAAGATGTCCTTCCAGCGGTGTGCTCGA
ACAGACAAGGGTGTGTCTGCAGCAGGGCAGGTGGTATCCCTAAAGGTGTGGCTGATTGATGACATTCTGG
ACAAGATCAACAGCCACCTTCCATCCCACATTCGGATTCTGGGATTGAAGAGGGTCACAGGCGGGTTCAA
CTCCAAGAACAAGTGTGACGCCAGGACCTACTGCTACATGCTGCCCACCTTTGCCTTTGCTCACAAGGAC
CGGGATGTACAGGACGAGAGCTATCGCCTGAGTGCAGAGACCCTGCAGCAGGTCAACCGCCTTCTGGCCT
GCTACAAAGGCACCCATAACTTCCATAACTTCACCTCGCAGAAGGGCCCAAGAGAGCCCAGTGCGCGCCG
CTACATTCTGGAGATGTACTGCGAAGAGCCCTTCGTACGTGAGGGCCTGGAGTTTGCTGTGATCAAGGTG
AAGGGCCAGAGCTTCATGATGCACCAGATCCGGAAGATGGTTGGCCTGGTGGTAGCCATTGTGAAGGGTT
ATGCCCCTGAGAGTGTCCTGGAGCGCAGCTGGGGAGAGGAGAAGGTGGATGTGCCCAAGGCACCCGGGCT
TGGCTTAGTCCTGGAGAGGGTTCACTTTGAAAAGTACAATCAACGCTTTGGCGGTGACGGGCTGCATGAG
CCCCTGGACTGGACGCAGGAGGAAGGGAAGGTGACTGCTTTCAAGGAGCAGTACATTTACCCTACCATTG
TCAGCACTGAGCGGGATGAGCGGTCCATGGCCCAGTGGCTCAATACTTTGCCGATCCACAACTTCAGTGG
CACTGCACTTGGAGCGGCTGACACAGGTGCCAAGGTCCCCAGTTCCCTGGAGGGCAGTGAAGGGGATGGA
GACACTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206726 protein sequence
Red=Cloning site Green=Tags(s)

MGFPRLWAALLRNWGRWTARPGPRVPGLPPMAGNKVPPALASHQPDRKGRGGWVWEETEHPAKRVKGGED
EEPPRKLPKRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRCAR
TDKGVSAAGQVVSLKVWLIDDILDKINSHLPSHIRILGLKRVTGGFNSKNKCDARTYCYMLPTFAFAHKD
RDVQDESYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARRYILEMYCEEPFVREGLEFAVIKV
KGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGEEKVDVPKAPGLGLVLERVHFEKYNQRFGGDGLHE
PLDWTQEEGKVTAFKEQYIYPTIVSTERDERSMAQWLNTLPIHNFSGTALGAADTGAKVPSSLEGSEGDG
DTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001025562
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1535 bp
RefSeq ORF 1182 bp
Locus ID 56361
Cytogenetics 5 F
MW 47.5 kDa

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