Ap1m1 (NM_007456) Mouse Tagged ORF Clone

CAT#: MR206741

Ap1m1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-1, mu subunit 1 (Ap1m1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ap1m1
• Synonyms: AA408894; Adtm1A; AP47; Cltnm; mu1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206741 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGCGCCGTCTACGTACTGGATCTGAAGGGCAAGGTGCTCATCTGCAGGAACTACCGTGGGG
  ATGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATTCTGATGGAGAAGGAGGAGGAGGGCATGCTGTC
  ACCTATCTTGGCCCATGGTGGCGTTCGTTTCATGTGGATTAAGCACAACAACCTGTACCTGGTCGCCACT
  TCAAAAAAGAATGCTTGTGTGTCACTGGTGTTCTCCTTCCTCTACAAGGTGGTACAGGTCTTCTCCGAGT
  ACTTTAAGGAGTTGGAGGAGGAGAGCATCCGAGACAACTTTGTCATCATCTACGAGCTGCTAGATGAGCT
  CATGGACTTTGGCTACCCGCAGACCACTGACAGCAAGATCTTGCAGGAGTACATCACTCAGGAAGGCCAC
  AAGCTGGAAACGGGGGCCCCTAGGCCCCCAGCCACAGTCACCAATGCTGTGTCCTGGCGTTCAGAAGGCA
  TCAAGTATCGGAAGAATGAAGTATTCCTGGATGTCATTGAGGCTGTTAACCTCTTGGTCAGTGCCAATGG
  CAACGTGCTGCGCAGTGAGATTGTGGGTTCCATCAAGATGCGGGTCTTCCTCTCAGGCATGCCTGAGTTA
  CGCCTGGGTCTCAATGACAAGGTCCTCTTCGACAACACAGGCCGAGGGAAGAGCAAGTCAGTGGAGCTGG
  AGGATGTGAAATTCCACCAGTGTGTGCGGCTGTCACGTTTTGAGAACGACCGCACTATCTCCTTCATCCC
  ACCCGACGGAGAGTTTGAACTCATGTCCTACCGCCTCAACACCCATGTGAAGCCTTTGATCTGGATTGAG
  TCCGTGATTGAGAAGCATTCCCACAGCCGCATTGAGTACATGGTCAAGGCCAAGAGCCAGTTCAAGAGGC
  GGTCAACAGCCAACAATGTAGAGATCCATATACCAGTCCCCAACGATGCTGATTCACCCAAGTTCAAGAC
  TACAGTGGGGAGTGTCAAGTGGGTCCCTGAAAACAGTGAGATCGTGTGGTCCGTCAAGTCCTTTCCGGGT
  GGCAAGGAGTACCTGATGCGGGCCCACTTTGGCCTTCCCAGTGTGGAAGCTGAAGACAAGGAGGGAAAGC
  CCCCCATCAGCGTCAAGTTTGAGATCCCCTATTTCACTACCTCTGGCATCCAGGTGCGCTACCTGAAAAT
  CATTGAGAAGAGTGGGTACCAGGCCCTGCCCTGGGTACGATATATCACACAGAACGGAGATTATCAGCTC
  CGGACCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206741 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVAT
  SKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGH
  KLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIEAVNLLVSANGNVLRSEIVGSIKMRVFLSGMPEL
  RLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIE
  SVIEKHSHSRIEYMVKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSVKSFPG
  GKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQL
  RTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007456
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007456.1, NM_007456.2, NM_007456.3, NM_007456.4, NM_007456.5, NP_031482.1
• RefSeq Size: 1964
• RefSeq ORF: 1272
• Locus ID: 11767
• MW: 48.5 kDa
• Gene Summary: This gene encodes the mu-1 subunit of the scaffolding adapter protein complex AP-1 and is a member of the mu adaptin family. The AP-1 complex, which consists of 4 subunits (mu-adaptin, beta-prime adaptin, gamma-adaptin, and the small chain adaptin), is one of the predominant coat proteins of membrane vesicles involved in eukaryotic post-Golgi trafficking. The AP-1 complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. AP-1 complex subunit mu-1 and other mu-adaptins select cargo proteins bearing sequence-specific sorting motifs. [provided by RefSeq, Jul 2016]